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Literature summary extracted from

  • Blazy, B.; Bardet, M.; Baudras, A.
    A standard high-yield purification of L-(+)-lactate: cytochrome c oxidoreductase (cytochrome b2) from the yeast Hansenula anomala (1978), Anal. Biochem., 88, 624-633.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.2.3 PMSF stabilizes, purification in absence of PMSF yields cleaved form of enzyme Wickerhamomyces anomalus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.2.3 Fe2+ bound to enzyme Wickerhamomyces anomalus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.2.3 62750
-
 4 * 62750, SDS-PAGE Wickerhamomyces anomalus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.2.3 (S)-lactate + 2 ferricytochrome c Wickerhamomyces anomalus
-
 pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.3 Wickerhamomyces anomalus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.2.3
-
 Wickerhamomyces anomalus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.2.3 895
-
 purified enzyme Wickerhamomyces anomalus

Storage Stability

EC Number Storage Stability Organism
1.1.2.3 4°C, saturated ammonium sulfate, under nitrogen, protected from light, stabe Wickerhamomyces anomalus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.3 (S)-lactate + 2 ferricytochrome c
-
 Wickerhamomyces anomalus pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?
1.1.2.3 additional information electron acceptors other than ferricytochrome c used Wickerhamomyces anomalus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.2.3 tetramer 4 * 62750, SDS-PAGE Wickerhamomyces anomalus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.3 additional information electron acceptors other than ferricytochrome c used Wickerhamomyces anomalus