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Literature summary extracted from

  • Keiner, R.; Kaiser, H.; Nakajima, K.; Hashimoto, T.; Draeger, B.
    Molecular cloning, expression and characterization of tropinone reductase II, an enzyme of the SDR family in Solanum tuberosum (L.) (2002), Plant Mol. Biol., 48, 299-308.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.236 cDNA encoding tropinone reductase II Hyoscyamus niger
1.1.1.236 cDNA homologous to pseudotropine-forming tropinone reductase isolated, recombinant protein functionally expressed in Escherichia coli BL21(DE3), transformed with pET21d vectors Solanum tuberosum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.236
-
 Solanum tuberosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.236 0.025
-
 Nortropinone
-
 Solanum tuberosum
1.1.1.236 0.033
-
 tropinone pH 6.4 Solanum tuberosum
1.1.1.236 0.052
-
 tropinone pH 7.6 Solanum tuberosum
1.1.1.236 0.111
-
 tropinone pH 5.0 Solanum tuberosum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.236 tropinone + NADPH + H+ Solanum tuberosum
-
 pseudotropine + NADP+
-
 ?
1.1.1.236 tropinone + NADPH + H+ Datura stramonium
-
 pseudotropine + NADP+
-
 ?
1.1.1.236 tropinone + NADPH + H+ Atropa belladonna
-
 pseudotropine + NADP+
-
 ?
1.1.1.236 tropinone + NADPH + H+ Hyoscyamus niger
-
 pseudotropine + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.236 Atropa belladonna
-
-
-
1.1.1.236 Datura stramonium
-
-
-
1.1.1.236 Hyoscyamus niger
-
-
-
1.1.1.236 Solanum tuberosum
-
 potato, cvs. Liu and Desiree
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.236
-
 Hyoscyamus niger
1.1.1.236 recombinant StTRII L1 Solanum tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.236 dormant eye
-
 Solanum tuberosum
-
1.1.1.236 flower
-
 Solanum tuberosum
-
1.1.1.236 flower
-
 Atropa belladonna
-
1.1.1.236 fruit
-
 Solanum tuberosum
-
1.1.1.236 leaf
-
 Solanum tuberosum
-
1.1.1.236 leaf
-
 Atropa belladonna
-
1.1.1.236 pith
-
 Solanum tuberosum
-
1.1.1.236 root
-
 Solanum tuberosum
-
1.1.1.236 root
-
 Datura stramonium
-
1.1.1.236 root
-
 Atropa belladonna
-
1.1.1.236 root mostly localized to the endodermis, less abundant in the pericycle and cortex Hyoscyamus niger
-
1.1.1.236 sprout
-
 Solanum tuberosum
-
1.1.1.236 stem
-
 Solanum tuberosum
-
1.1.1.236 stem
-
 Datura stramonium
-
1.1.1.236 stem
-
 Atropa belladonna
-
1.1.1.236 stolon
-
 Solanum tuberosum
-
1.1.1.236 tuber
-
 Solanum tuberosum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.236 nortropinone + NADPH
-
 Solanum tuberosum ? + NADP+
-
 ?
1.1.1.236 tropinone + NADPH + H+
-
 Solanum tuberosum pseudotropine + NADP+
-
 ?
1.1.1.236 tropinone + NADPH + H+
-
 Solanum tuberosum pseudotropine + NADP+
-
 ir
1.1.1.236 tropinone + NADPH + H+
-
 Datura stramonium pseudotropine + NADP+
-
 ?
1.1.1.236 tropinone + NADPH + H+
-
 Datura stramonium pseudotropine + NADP+
-
 ir
1.1.1.236 tropinone + NADPH + H+
-
 Atropa belladonna pseudotropine + NADP+
-
 ?
1.1.1.236 tropinone + NADPH + H+
-
 Hyoscyamus niger pseudotropine + NADP+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.236 NADPH
-
 Solanum tuberosum
1.1.1.236 NADPH
-
 Datura stramonium
1.1.1.236 NADPH
-
 Hyoscyamus niger