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Literature summary extracted from

  • Nakajima, K.; Hashimoto, T.
    Two tropinone reductases, that catalyze opposite stereospecific reductions in tropane alkaloid biosynthesis, are localized in plant root with different cell-specific patterns (1999), Plant Cell Physiol., 40, 1099-1107.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.236 TRII cDNA, in Escherichia coli expression vector pET21d Datura stramonium
1.1.1.236 TRII cDNA, in Escherichia coli expression vector pET21d Hyoscyamus niger

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.236
-
Datura stramonium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.236 29000
-
3 * 29000, SDS-PAGE Hyoscyamus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.236 tropinone + NADPH + H+ Datura stramonium
-
pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+ Atropa belladonna
-
pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+ Hyoscyamus niger
-
pseudotropine + NADP+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.236 Atropa belladonna
-
-
-
1.1.1.236 Datura stramonium
-
-
-
1.1.1.236 Hyoscyamus niger
-
-
-
1.1.1.236 no activity in Nicotiana tabacum
-
tobacco
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.236
-
Datura stramonium
1.1.1.236
-
Hyoscyamus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.236 endodermis
-
Hyoscyamus niger
-
1.1.1.236 root
-
Datura stramonium
-
1.1.1.236 root
-
Atropa belladonna
-
1.1.1.236 root
-
Hyoscyamus niger
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.236 tropinone + NADPH + H+
-
Datura stramonium pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+
-
Atropa belladonna pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+
-
Hyoscyamus niger pseudotropine + NADP+
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.236 trimer
-
Datura stramonium
1.1.1.236 trimer
-
Atropa belladonna
1.1.1.236 trimer 3 * 29000, SDS-PAGE Hyoscyamus niger