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Literature summary extracted from

  • Portsteffen, A.; Draeger, B.; Nahrstedt, A.
    Two tropinone reducing enzymes from Datura stramonium transformed root cultures (1992), Phytochemistry, 31, 1135-1138.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.1.1.236 activity rapidly lost during purification Datura stramonium
1.1.1.236 activity rapidly lost during purification Atropa belladonna
1.1.1.236 activity rapidly lost during purification Hyoscyamus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.236 p-chloromercuribenzoic acid
-
Hyoscyamus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.236 0.0061
-
NADPH
-
Hyoscyamus niger
1.1.1.236 0.0076
-
NADH
-
Hyoscyamus niger
1.1.1.236 0.017
-
NADPH
-
Datura stramonium
1.1.1.236 0.0211
-
NADPH
-
Hyoscyamus niger
1.1.1.236 0.023
-
NADPH
-
Datura stramonium
1.1.1.236 0.034
-
tropinone
-
Hyoscyamus niger
1.1.1.236 0.0351
-
tropinone
-
Hyoscyamus niger
1.1.1.236 0.22
-
tropinone
-
Datura stramonium
1.1.1.236 0.251
-
NADP+
-
Hyoscyamus niger
1.1.1.236 0.265
-
N-propylyl-4-piperidone
-
Hyoscyamus niger
1.1.1.236 0.534
-
4-ethylcyclohexanone
-
Hyoscyamus niger
1.1.1.236 0.77
-
N-Methyl-4-piperidone
-
Hyoscyamus niger
1.1.1.236 0.83
-
tropinone
-
Datura stramonium
1.1.1.236 2.03
-
4-methylcyclohexanone
-
Hyoscyamus niger
1.1.1.236 7.58
-
3-methylcyclohexanone
-
Hyoscyamus niger
1.1.1.236 21.6
-
4-Piperidone
-
Hyoscyamus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.236 25000 55000 TRII preparation is not yet pure and shows numerous bands Datura stramonium
1.1.1.236 29000
-
3 * 29000, SDS-PAGE Hyoscyamus niger
1.1.1.236 29000
-
4 * 29000, SDS-PAGE Hyoscyamus niger
1.1.1.236 69000
-
minor peak, gel filtration Hyoscyamus niger
1.1.1.236 103000
-
major peak, gel filtration Hyoscyamus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.236 tropinone + NADPH + H+ Datura inoxia
-
pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+ Datura stramonium
-
pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+ Atropa belladonna
-
pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+ Hyoscyamus muticus
-
pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+ Hyoscyamus pusillus
-
pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+ Hyoscyamus canariensis
-
pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+ Hyoscyamus bohemicus
-
pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+ Physochlaina orientalis
-
pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+ Physalis philadelphica
-
pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+ Physalis edulis
-
pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+ Calystegia sepium
-
pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+ Alkekengi officinarum
-
pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+ Hyoscyamus niger reverse reaction very inefficient, about 1.3% of the forward activity pseudotropine + NADP+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.236 Alkekengi officinarum
-
-
-
1.1.1.236 Atropa belladonna
-
-
-
1.1.1.236 Calystegia sepium
-
-
-
1.1.1.236 Datura inoxia
-
-
-
1.1.1.236 Datura stramonium
-
-
-
1.1.1.236 Hyoscyamus bohemicus
-
-
-
1.1.1.236 Hyoscyamus canariensis
-
-
-
1.1.1.236 Hyoscyamus muticus
-
-
-
1.1.1.236 Hyoscyamus niger
-
-
-
1.1.1.236 Hyoscyamus pusillus
-
-
-
1.1.1.236 no activity in Brassica campestris
-
-
-
1.1.1.236 no activity in Browallia americana
-
-
-
1.1.1.236 no activity in Nicotiana tabacum
-
tobacco
-
1.1.1.236 Physalis edulis
-
-
-
1.1.1.236 Physalis philadelphica
-
-
-
1.1.1.236 Physochlaina orientalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.236
-
Datura stramonium
1.1.1.236
-
Hyoscyamus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.236 flower
-
Atropa belladonna
-
1.1.1.236 leaf
-
Atropa belladonna
-
1.1.1.236 root
-
Datura inoxia
-
1.1.1.236 root
-
Atropa belladonna
-
1.1.1.236 root
-
Hyoscyamus niger
-
1.1.1.236 root
-
Physalis philadelphica
-
1.1.1.236 root
-
Physalis edulis
-
1.1.1.236 root
-
Calystegia sepium
-
1.1.1.236 root
-
Alkekengi officinarum
-
1.1.1.236 root hairy roots Datura stramonium
-
1.1.1.236 stem
-
Datura stramonium
-
1.1.1.236 stem
-
Atropa belladonna
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.236 7.79
-
-
Datura stramonium

Storage Stability

EC Number Storage Stability Organism
1.1.1.236 -20°C, stable for at least 3 months Datura stramonium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.236 2-methyl-cyclohexanone + NADPH
-
Hyoscyamus niger 2-methylcyclohexanol + NADP+
-
?
1.1.1.236 3-methyl-cyclohexanone + NADPH
-
Hyoscyamus niger 3-methylcyclohexanol + NADP+
-
?
1.1.1.236 4-ethyl-cyclohexanone + NADPH
-
Datura stramonium 4-ethylcyclohexanol + NADP+
-
?
1.1.1.236 4-ethyl-cyclohexanone + NADPH
-
Hyoscyamus niger 4-ethylcyclohexanol + NADP+
-
?
1.1.1.236 4-methyl-cyclohexanone + NADPH
-
Datura stramonium 4-methylcyclohexanol + NADP+
-
?
1.1.1.236 4-methyl-cyclohexanone + NADPH
-
Hyoscyamus niger 4-methylcyclohexanol + NADP+
-
?
1.1.1.236 4-piperidone + NADPH
-
Hyoscyamus niger piperidine-4-ol + NADP+
-
?
1.1.1.236 6-hydroxytropinone + NADPH
-
Datura stramonium ? + NADP+
-
?
1.1.1.236 6-hydroxytropinone + NADPH
-
Hyoscyamus niger ? + NADP+
-
?
1.1.1.236 N-methyl-4-piperidone + NADPH
-
Datura stramonium N-methylpiperidin-4-ol + NADP+
-
?
1.1.1.236 N-methyl-4-piperidone + NADPH
-
Hyoscyamus niger N-methylpiperidin-4-ol + NADP+
-
?
1.1.1.236 N-propyl-4-piperidone + NADPH
-
Datura stramonium N-propylpiperidin-4-ol + NADP+
-
?
1.1.1.236 N-propyl-4-piperidone + NADPH
-
Hyoscyamus niger N-propylpiperidin-4-ol + NADP+
-
?
1.1.1.236 tetrahydrothiopyran-4-one + NADPH
-
Datura stramonium tetrahydrothiopyran-4-ol + NADP+
-
?
1.1.1.236 tetrahydrothiopyran-4-one + NADPH
-
Hyoscyamus niger tetrahydrothiopyran-4-ol + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+
-
Datura inoxia pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+
-
Datura stramonium pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+
-
Atropa belladonna pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+
-
Hyoscyamus niger pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+
-
Hyoscyamus muticus pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+
-
Hyoscyamus pusillus pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+
-
Hyoscyamus canariensis pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+
-
Hyoscyamus bohemicus pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+
-
Physochlaina orientalis pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+
-
Physalis philadelphica pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+
-
Physalis edulis pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+
-
Calystegia sepium pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+
-
Alkekengi officinarum pseudotropine + NADP+
-
?
1.1.1.236 tropinone + NADPH + H+ reverse reaction very inefficient, about 1.3% of the forward activity Hyoscyamus niger pseudotropine + NADP+
-
r

Subunits

EC Number Subunits Comment Organism
1.1.1.236 tetramer 4 * 29000, SDS-PAGE Hyoscyamus niger
1.1.1.236 trimer
-
Datura stramonium
1.1.1.236 trimer
-
Atropa belladonna
1.1.1.236 trimer 3 * 29000, SDS-PAGE Hyoscyamus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.236 5.3 6.5
-
Hyoscyamus niger
1.1.1.236 5.8
-
forward reaction Hyoscyamus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.236 4 5 reverse oxidative activities Hyoscyamus niger
1.1.1.236 4.5 8.5 tolerant to pH changes over a wide range Datura stramonium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.236 5.8 8.5 more acidic buffers inactivate the enzyme highly, 8% of the maximum activity at pH 4.5, more alkaline buffers inhibit the activity less strongly, 61% of the maximum activity at pH 8.5 Hyoscyamus niger

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.236 NADPH
-
Hyoscyamus niger
1.1.1.236 NADPH activity exclusively with NADPH, not active with NADH Datura stramonium