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Literature summary extracted from
- Two tropinone reductases with distinct stereospecificities from cultured roots of Hyoscyamus niger (1992), Plant Physiol., 100, 836-845.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.206 | 8-thiabicyclo(3,2,1)octane-3-one | complete inhibition at 4 mM | Hyoscyamus niger |  |
| 1.1.1.206 | Ca2+ | 0-15% inactivation at 1 mM | Hyoscyamus niger | |
| 1.1.1.206 | Cd2+ | 26-43% inactivation at 1 mM | Hyoscyamus niger | |
| 1.1.1.206 | Co2+ | 0-15% inactivation at 1 mM | Hyoscyamus niger | |
| 1.1.1.206 | Cu2+ | 99% inactivation at 1 mM | Hyoscyamus niger | |
| 1.1.1.206 | diethyl dicarbonate | complete inhibition at 0.1 mM | Hyoscyamus niger | |
| 1.1.1.206 | Fe2+ | 0-15% inactivation at 1 mM | Hyoscyamus niger | |
| 1.1.1.206 | Fe3+ | 0-15% inactivation at 1 mM | Hyoscyamus niger | |
| 1.1.1.206 | Hg2+ | 99% inactivation at 1 mM | Hyoscyamus niger | |
| 1.1.1.206 | Li+ | 0-15% inactivation at 1 mM | Hyoscyamus niger | |
| 1.1.1.206 | Mg2+ | 0-15% inactivation at 1 mM | Hyoscyamus niger | |
| 1.1.1.206 | Mn2+ | 0-15% inactivation at 1 mM | Hyoscyamus niger | |
| 1.1.1.206 | additional information | not inhibited by EDTA and iodoacetamide | Hyoscyamus niger | |
| 1.1.1.206 | Ni2+ | 0-15% inactivation at 1 mM | Hyoscyamus niger | |
| 1.1.1.206 | p-chloromercuribenzoate | strong inhibition at 0.1 and 1.0 mM | Hyoscyamus niger | |
| 1.1.1.206 | Zn2+ | 0-15% inactivation at 1 mM | Hyoscyamus niger | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.206 | 0.001 | - |
4-methylcyclohexanone | - |
Hyoscyamus niger | |
| 1.1.1.206 | 0.003 | - |
4-ethylcyclohexanone | - |
Hyoscyamus niger | |
| 1.1.1.206 | 0.003 | - |
8-thiabicyclo(3,2,1)octane-3-one | - |
Hyoscyamus niger | |
| 1.1.1.206 | 0.004 | - |
3-methylcyclohexanone | - |
Hyoscyamus niger | |
| 1.1.1.206 | 0.011 | - |
NADPH | - |
Hyoscyamus niger | |
| 1.1.1.206 | 0.042 | - |
NADP+ | - |
Hyoscyamus niger | |
| 1.1.1.206 | 0.231 | - |
N-Methyl-4-piperidone | - |
Hyoscyamus niger | |
| 1.1.1.206 | 1.01 | - |
tropinone | - |
Hyoscyamus niger | |
| 1.1.1.206 | 1.81 | - |
3-quinuclidinone | - |
Hyoscyamus niger | |
| 1.1.1.206 | 2.6 | - |
Tropine | - |
Hyoscyamus niger | |
| 1.1.1.206 | 10 | - |
NADH | - |
Hyoscyamus niger | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.206 | 30000 | - |
SDS-PAGE | Hyoscyamus niger |
| 1.1.1.206 | 115000 | - |
gel filtration | Hyoscyamus niger |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.236 | tropinone + NADPH + H+ | Hyoscyamus niger | - |
pseudotropine + NADP+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.206 | Atropa acuminata | - |
- |
- |
| 1.1.1.206 | Atropa belladonna | - |
- |
- |
| 1.1.1.206 | Datura stramonium | - |
- |
- |
| 1.1.1.206 | Duboisia hopwoodii | - |
- |
- |
| 1.1.1.206 | Duboisia leichhardtii | - |
- |
- |
| 1.1.1.206 | Hyoscyamus albus | - |
- |
- |
| 1.1.1.206 | Hyoscyamus bohemicus | - |
- |
- |
| 1.1.1.206 | Hyoscyamus canariensis | - |
- |
- |
| 1.1.1.206 | Hyoscyamus muticus | - |
- |
- |
| 1.1.1.206 | Hyoscyamus niger | - |
and strain Hn11 | - |
| 1.1.1.206 | Hyoscyamus niger Hn | - |
and strain Hn11 | - |
| 1.1.1.206 | Hyoscyamus pusillus | - |
- |
- |
| 1.1.1.206 | no activity in Brassica campestris | - |
- |
- |
| 1.1.1.206 | no activity in Browallia americana | - |
- |
- |
| 1.1.1.206 | no activity in Calystegia sepium | - |
- |
- |
| 1.1.1.206 | no activity in Nicotiana tabacum | - |
- |
- |
| 1.1.1.206 | no activity in Physalis alkekengi | - |
- |
- |
| 1.1.1.206 | Physalis edulis | - |
- |
- |
| 1.1.1.206 | Physalis philadelphica | - |
- |
- |
| 1.1.1.206 | Physochlaina orientalis | - |
- |
- |
| 1.1.1.236 | Hyoscyamus niger | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.206 | - |
Hyoscyamus niger |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.206 | leaf | - |
Datura stramonium | - |
| 1.1.1.206 | root | - |
Datura stramonium | - |
| 1.1.1.206 | root | - |
Atropa belladonna | - |
| 1.1.1.206 | root | - |
Hyoscyamus niger | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.206 | 0.6 | - |
tropinone | Hyoscyamus albus |
| 1.1.1.206 | 1.2 | - |
tropinone | Hyoscyamus bohemicus |
| 1.1.1.206 | 1.2 | - |
tropinone | Atropa acuminata |
| 1.1.1.206 | 3 | - |
tropinone | Physochlaina orientalis |
| 1.1.1.206 | 7.5 | - |
tropinone, strain Hn | Hyoscyamus niger |
| 1.1.1.206 | 7.5 | - |
tropinone | Atropa belladonna |
| 1.1.1.206 | 7.5 | - |
tropinone | Hyoscyamus canariensis |
| 1.1.1.206 | 10.2 | - |
tropinone | Hyoscyamus pusillus |
| 1.1.1.206 | 10.8 | - |
tropinone | Hyoscyamus muticus |
| 1.1.1.206 | 15 | - |
tropinone, strain Hn11 | Hyoscyamus niger |
| 1.1.1.206 | 30 | - |
tropinone | Duboisia leichhardtii |
| 1.1.1.206 | 30 | - |
tropinone | Duboisia hopwoodii |
| 1.1.1.206 | 45 | - |
tropinone | Datura stramonium |
| 1.1.1.206 | 54 | - |
tropinone | Physalis edulis |
| 1.1.1.206 | 66 | - |
tropinone | Physalis philadelphica |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.206 | 4°C, NADPH, NADP+ and glycerol enhance stability | Hyoscyamus niger |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.206 | 3-methylcyclohexanone + NADPH | - |
Datura stramonium | 3-methylcyclohexanol + NADP+ | - |
? | |
| 1.1.1.206 | 3-methylcyclohexanone + NADPH | - |
Hyoscyamus niger | 3-methylcyclohexanol + NADP+ | - |
? | |
| 1.1.1.206 | 3-methylcyclohexanone + NADPH | - |
Hyoscyamus niger Hn | 3-methylcyclohexanol + NADP+ | - |
? | |
| 1.1.1.206 | 3-quinuclidinone + NADPH + H+ | - |
Datura stramonium | quinuclidin-3-ol + NADP+ | - |
? | |
| 1.1.1.206 | 3-quinuclidinone + NADPH + H+ | - |
Hyoscyamus niger | quinuclidin-3-ol + NADP+ | - |
? | |
| 1.1.1.206 | 4-ethylcyclohexanone + NADPH | - |
Datura stramonium | 4-ethylcyclohexanol + NADP+ | - |
? | |
| 1.1.1.206 | 4-ethylcyclohexanone + NADPH | - |
Hyoscyamus niger | 4-ethylcyclohexanol + NADP+ | - |
? | |
| 1.1.1.206 | 4-ethylcyclohexanone + NADPH | - |
Hyoscyamus niger Hn | 4-ethylcyclohexanol + NADP+ | - |
? | |
| 1.1.1.206 | 4-methylcyclohexanone + NADPH | - |
Datura stramonium | 4-methylcyclohexanol + NADP+ | - |
? | |
| 1.1.1.206 | 4-methylcyclohexanone + NADPH | - |
Hyoscyamus niger | 4-methylcyclohexanol + NADP+ | - |
? | |
| 1.1.1.206 | 4-methylcyclohexanone + NADPH | - |
Hyoscyamus niger Hn | 4-methylcyclohexanol + NADP+ | - |
? | |
| 1.1.1.206 | 8-thiabicyclo(3,2,1)octane-3-one + NADPH | - |
Hyoscyamus niger | 8-thiabicyclo(3,2,1)octane-3-ol + NADP+ | - |
? | |
| 1.1.1.206 | N-methyl-4-piperidone + NADPH | - |
Datura stramonium | 1-methylpiperidin-4-ol + NADP+ | - |
? | |
| 1.1.1.206 | N-methyl-4-piperidone + NADPH | - |
Hyoscyamus niger | 1-methylpiperidin-4-ol + NADP+ | - |
? | |
| 1.1.1.206 | N-methyl-4-piperidone + NADPH | - |
Hyoscyamus niger Hn | 1-methylpiperidin-4-ol + NADP+ | - |
? | |
| 1.1.1.206 | tropine + NADP+ | - |
Datura stramonium | tropinone + NADPH | - |
r | |
| 1.1.1.206 | tropine + NADP+ | - |
Hyoscyamus niger | tropinone + NADPH | - |
r | |
| 1.1.1.206 | tropine + NADP+ | - |
Hyoscyamus niger Hn | tropinone + NADPH | - |
r | |
| 1.1.1.206 | tropinone + NADH + H+ | - |
Hyoscyamus niger | tropine + NAD+ | - |
r | |
| 1.1.1.236 | tropinone + NADPH + H+ | - |
Hyoscyamus niger | pseudotropine + NADP+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.206 | tetramer | 4 * 30000, gel filtration | Hyoscyamus niger |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.206 | 6.1 | - |
tropinone reduction, narrow pH-range | Hyoscyamus niger |
| 1.1.1.206 | 7.6 | - |
tropine oxidation | Hyoscyamus niger |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.206 | NADH | lower affinity than with NADPH | Hyoscyamus niger | |
| 1.1.1.206 | NADPH | - |
Datura stramonium | |
| 1.1.1.206 | NADPH | - |
Hyoscyamus niger | |
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