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Literature summary extracted from

  • Couladis, M.M.; Brent Friesen, J.; Landgrebe, M.E.; Leete, E.
    Enzymes catalysing the reduction of tropinone to tropine and psi-tropine isolated from the roots of Datura innoxia (1991), Phytochemistry, 30, 801-805.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.1.1.236 denatured enzyme does not catalyze the reduction of tropinone with NADPH Datura inoxia
1.1.1.236 very unstable Datura stramonium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.236 tropinone + NADPH + H+ Datura inoxia
-
 pseudotropine + NADP+
-
 ?
1.1.1.236 tropinone + NADPH + H+ Datura stramonium
-
 pseudotropine + NADP+
-
 ?
1.1.1.236 tropinone + NADPH + H+ Atropa belladonna
-
 pseudotropine + NADP+
-
 ?
1.1.1.236 tropinone + NADPH + H+ Hyoscyamus niger
-
 pseudotropine + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.236 Atropa belladonna
-
-
-
1.1.1.236 Datura inoxia
-
-
-
1.1.1.236 Datura stramonium
-
-
-
1.1.1.236 Hyoscyamus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.236
-
 Datura inoxia
1.1.1.236
-
 Hyoscyamus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.236 root
-
 Datura inoxia
-
1.1.1.236 root
-
 Datura stramonium
-
1.1.1.236 root
-
 Atropa belladonna
-
1.1.1.236 root
-
 Hyoscyamus niger
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.236 0.0145
-
-
 Datura inoxia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.236 N-methyl-4-piperidone + NADPH
-
 Datura stramonium N-methylpiperidin-4-ol + NADP+
-
 ?
1.1.1.236 N-methyl-4-piperidone + NADPH more efficient substrate, 230% of activity with tropinone Hyoscyamus niger N-methylpiperidin-4-ol + NADP+
-
 ?
1.1.1.236 N-methyltropinone + NADPH
-
 Datura inoxia N-methyl-3-beta-tropine + NADP+
-
 ir
1.1.1.236 N-propyl-4-piperidone + NADPH
-
 Datura stramonium N-propylpiperidin-4-ol + NADP+
-
 ?
1.1.1.236 N-propyl-4-piperidone + NADPH more efficient substrate, 300% of activity with tropinone Hyoscyamus niger N-propylpiperidin-4-ol + NADP+
-
 ?
1.1.1.236 tropine + NADP+
-
 Datura stramonium tropinone + NADPH
-
 r
1.1.1.236 tropinone + NADPH + H+
-
 Datura inoxia pseudotropine + NADP+
-
 ?
1.1.1.236 tropinone + NADPH + H+
-
 Datura stramonium pseudotropine + NADP+
-
 ?
1.1.1.236 tropinone + NADPH + H+
-
 Datura stramonium pseudotropine + NADP+
-
 ir
1.1.1.236 tropinone + NADPH + H+
-
 Atropa belladonna pseudotropine + NADP+
-
 ?
1.1.1.236 tropinone + NADPH + H+
-
 Hyoscyamus niger pseudotropine + NADP+
-
 ?
1.1.1.236 tropinone + NADPH + H+ enzymes fails to catalyze the oxidation of tropine to tropinone in presence of NADP+ Datura inoxia pseudotropine + NADP+
-
 ir
1.1.1.236 tropinone + NADPH + H+
-
 Datura stramonium tropine + NADP+ only tropine formed by the reduction of tropinone, no evidence for the formation of psi-tropine r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.236 60
-
 tropine oxidation Datura stramonium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.236 6
-
-
 Hyoscyamus niger
1.1.1.236 6.4
-
-
 Datura inoxia
1.1.1.236 6.8
-
 tropinone reduction Datura stramonium
1.1.1.236 9.5
-
 tropine oxidation Datura stramonium

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.236 NADH less effective by a factor of 3.7 than NADPH Datura stramonium
1.1.1.236 NADH preference for NADPH, velocity 5.4% of the velocity found with NADPH Hyoscyamus niger
1.1.1.236 NADH 70-80% reduction in psi tropine forming reductase activity when NADH is substituted for NADPH Datura inoxia
1.1.1.236 NADP+ oxidation does not occur when NAD+ is substituted for NADP+ Datura stramonium
1.1.1.236 NADPH
-
 Datura stramonium
1.1.1.236 NADPH
-
 Hyoscyamus niger