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Literature summary extracted from
- Purification and characterization of pseudotropine forming tropinone reductase from Hyoscyamus niger root cultures (1988), Agric. Biol. Chem., 52, 2663-2667.
No PubMed abstract available
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.236 | 0.0211 | - |
NADPH | - |
Hyoscyamus niger |  |
| 1.1.1.236 | 0.0351 | - |
tropinone | - |
Hyoscyamus niger | |
| 1.1.1.236 | 0.057 | - |
N-Methyl-4-piperidone | - |
Hyoscyamus niger | |
| 1.1.1.236 | 0.299 | - |
N-propylyl-4-piperidone | - |
Hyoscyamus niger | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.236 | 39000 | - |
2 * 39000, SDS-PAGE | Hyoscyamus niger |
| 1.1.1.236 | 84000 | - |
gel filtration | Hyoscyamus niger |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.236 | tropinone + NADPH + H+ | Datura stramonium | - |
pseudotropine + NADP+ | - |
? | |
| 1.1.1.236 | tropinone + NADPH + H+ | Hyoscyamus niger | - |
pseudotropine + NADP+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.236 | Datura stramonium | - |
- |
- |
| 1.1.1.236 | Hyoscyamus niger | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.236 | - |
Hyoscyamus niger |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.236 | root | - |
Datura stramonium | - |
| 1.1.1.236 | root | - |
Hyoscyamus niger | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.236 | 1.05 | - |
- |
Hyoscyamus niger |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.236 | -20°C, enzyme preparation can be stored for 3 months without loss of activity | Hyoscyamus niger |
| 1.1.1.236 | 4°C, enzyme preparation can be stored for 4 days without loss of activity, when kept in 10% glycerol enzyme activity is lost within 2 days | Hyoscyamus niger |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.236 | 4-methyl-cyclohexanone + NADPH | - |
Datura stramonium | 4-methylcyclohexanol + NADP+ | - |
? | |
| 1.1.1.236 | 4-methyl-cyclohexanone + NADPH | - |
Hyoscyamus niger | 4-methylcyclohexanol + NADP+ | - |
? | |
| 1.1.1.236 | 4-piperidone + NADPH | - |
Hyoscyamus niger | piperidine-4-ol + NADP+ | - |
? | |
| 1.1.1.236 | 6-hydroxytropinone + NADPH | - |
Datura stramonium | ? + NADP+ | - |
? | |
| 1.1.1.236 | 6-hydroxytropinone + NADPH | - |
Hyoscyamus niger | ? + NADP+ | - |
? | |
| 1.1.1.236 | N-methyl-4-piperidone + NADPH | - |
Datura stramonium | N-methylpiperidin-4-ol + NADP+ | - |
? | |
| 1.1.1.236 | N-methyl-4-piperidone + NADPH | more efficient substrate, 230% of activity with tropinone | Hyoscyamus niger | N-methylpiperidin-4-ol + NADP+ | - |
? | |
| 1.1.1.236 | N-propyl-4-piperidone + NADPH | - |
Datura stramonium | N-propylpiperidin-4-ol + NADP+ | - |
? | |
| 1.1.1.236 | N-propyl-4-piperidone + NADPH | more efficient substrate, 300% of activity with tropinone | Hyoscyamus niger | N-propylpiperidin-4-ol + NADP+ | - |
? | |
| 1.1.1.236 | nortropinone + NADPH | - |
Hyoscyamus niger | ? + NADP+ | - |
? | |
| 1.1.1.236 | tetrahydrothiopyran-4-one + NADPH | - |
Datura stramonium | tetrahydrothiopyran-4-ol + NADP+ | - |
? | |
| 1.1.1.236 | tetrahydrothiopyran-4-one + NADPH | - |
Hyoscyamus niger | tetrahydrothiopyran-4-ol + NADP+ | - |
? | |
| 1.1.1.236 | tropinone + NADPH + H+ | - |
Datura stramonium | pseudotropine + NADP+ | - |
? | |
| 1.1.1.236 | tropinone + NADPH + H+ | - |
Hyoscyamus niger | pseudotropine + NADP+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.236 | dimer | - |
Datura stramonium |
| 1.1.1.236 | dimer | 2 * 39000, SDS-PAGE | Hyoscyamus niger |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.236 | 30 | - |
- |
Hyoscyamus niger |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.236 | 24 | 56 | 77% of the maximum activity at 24°C, completely inactivated at 56°C | Hyoscyamus niger |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.236 | 5.8 | - |
- |
Hyoscyamus niger |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.236 | 5.8 | 8.5 | more acidic buffers inactivate the enzyme highly, 8% of the maximum activity at pH 4.5, more alkaline buffers inhibit the activity less strongly, 61% of the maximum activity at pH 8.5 | Hyoscyamus niger |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.236 | NADH | - |
Datura stramonium | |
| 1.1.1.236 | NADH | preference for NADPH, velocity 5.4% of the velocity found with NADPH | Hyoscyamus niger | |
| 1.1.1.236 | NADPH | - |
Datura stramonium | |
| 1.1.1.236 | NADPH | - |
Hyoscyamus niger | |
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