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Literature summary extracted from

  • Delavault, P.; Simier, P.; Thoiron, S.; Veronesi, C.; Fer, A.; Thalouarn, P.
    Isolation of mannose 6-phosphate reductase cDNA, changes in enzyme activity and mannitol content in broomrape (Orobanche ramosa) parasitic on tomato roots (2002), Physiol. Plant., 115, 48-55.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.224 agriculture enzyme is a target for herbizide treatment Phelipanche ramosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.224
-
 Phelipanche ramosa
1.1.1.224 single gene, constitutive, DNA sequence determination and analysis, expression in Escherichia coli Phelipanche ramosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.224 35000
-
 theoretical molecular weight, calculated from cDNA sequence Phelipanche ramosa
1.1.1.224 37000
-
 1 * 37000, SDS-PAGE of recombinant enzyme Phelipanche ramosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.224 D-mannitol 1-phosphate + NADP+ Phelipanche ramosa key enzyme in mannitol production in the parasite D-mannose 6-phosphate + NADPH + H+
-
 ?
1.1.1.224 D-mannose 6-phosphate + NADPH Phelipanche ramosa key enzyme in mannitol production in higher plants ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.224 Phelipanche ramosa
-
 broomrape
-
1.1.1.224 Phelipanche ramosa
-
 parasitic broomrape on tomato roots
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.224 purification of recombinant enzyme Phelipanche ramosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.224 additional information overview over different developmental stages Phelipanche ramosa
-
1.1.1.224 additional information constitutive gene expression during different developmental stages, e.g. germination and procaulome growth, of the parasite attached to tomato roots, high activity during tubercle enlargement and crown root formation Phelipanche ramosa
-
1.1.1.224 root
-
 Phelipanche ramosa
-
1.1.1.224 seed
-
 Phelipanche ramosa
-
1.1.1.224 shoot
-
 Phelipanche ramosa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.224 additional information
-
 measurement of activity during plant development Phelipanche ramosa
1.1.1.224 0.00822
-
 crude extract from expression in Escherichia coli Phelipanche ramosa
1.1.1.224 0.12
-
 in tubercle Phelipanche ramosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.224 D-mannitol 1-phosphate + NADP+
-
 Phelipanche ramosa D-mannose 6-phosphate + NADPH + H+
-
 ?
1.1.1.224 D-mannitol 1-phosphate + NADP+ key enzyme in mannitol production in the parasite Phelipanche ramosa D-mannose 6-phosphate + NADPH + H+
-
 ?
1.1.1.224 D-mannose 6-phosphate + NADPH key enzyme in mannitol production in higher plants Phelipanche ramosa ?
-
 ?
1.1.1.224 D-mannose 6-phosphate + NADPH
-
 Phelipanche ramosa D-mannitol 1-phosphate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.224 monomer 1 * 37000, SDS-PAGE of recombinant enzyme Phelipanche ramosa

Synonyms

EC Number Synonyms Comment Organism
1.1.1.224 M6PR
-
 Phelipanche ramosa
1.1.1.224 mannose 6-phosphate reductase
-
 Phelipanche ramosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.224 NADP+
-
 Phelipanche ramosa
1.1.1.224 NADPH
-
 Phelipanche ramosa