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Literature summary extracted from

  • Singh, S.; McCallum, J.; Gruber, M.Y.; Towers, G.H.N.; Muir, A.D.; Bohm, B.A.; Koupai-Abyazani, M.R.; Glass, A.D.M.
    Biosynthesis of flavan-3-ols by leaf extracts of Onobrychis viciifolia (1997), Phytochemistry, 44, 425-432.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.219 diethyldithiocarbamate 50% inhibition at 2 mM Onobrychis viciifolia
1.1.1.219 dithiothreitol 50% inhibition at 5 mM Onobrychis viciifolia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.219 additional information Onobrychis viciifolia biosynthesis of proanthocyanidin polymers (condensed tannins) ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.219 Onobrychis viciifolia
-
 sainfoin, cv. Melrose
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.219 leaf
-
 Onobrychis viciifolia
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.219 additional information
-
 measurement of reductase activity during leaf development Onobrychis viciifolia

Storage Stability

EC Number Storage Stability Organism
1.1.1.219 5°C, stable for 36 h Onobrychis viciifolia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.219 (+)-dihydromyricetin + NADPH i.e. 5'-hydroxy-dihydroquercetin Onobrychis viciifolia cis-3,4-leucodelphinidin + NADP+ i.e. 5,7,4'-trihydroxyflavan-3,4-cis-diol ?
1.1.1.219 (+)-dihydromyricetin + NADPH i.e. 5'-hydroxy-dihydroquercetin Onobrychis viciifolia cis-3,4-leucodelphinidin + NADP+ configuration 2R,3S-trans-3S,4S-cis-leucodelphinidin ?
1.1.1.219 (+)-dihydroquercetin + NADPH
-
 Onobrychis viciifolia cis-3,4-leucocyanidin + NADP+ i.e. 5,7,3',4'-tetrahydroxyflavan-3,4-cis-diol, 2,3-trans-configuration retained ?
1.1.1.219 additional information biosynthesis of proanthocyanidin polymers (condensed tannins) Onobrychis viciifolia ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.219 30
-
-
 Onobrychis viciifolia

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.219 6.4 7.4
-
 Onobrychis viciifolia

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.219 NADH reduced activity compared to NADPH Onobrychis viciifolia
1.1.1.219 NADPH
-
 Onobrychis viciifolia