BRENDA - Enzyme Database

Flavan-3-ol biosynthesis. The conversion of (+)-dihydroquercetin and flavan-3,4-cis-diol (leucocyanidin) to (+)-catechin by reductases extracted from cell suspension cultures of douglas fir

Stafford, H.A.; Lester, H.H.; Plant Physiol. 76, 184-186 (1984)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.219
0.037
-
(+)-dihydroquercetin
estimation
Pseudotsuga menziesii
1.17.1.3
0.037
-
dihydroquercetin
pH 7.4
Pseudotsuga menziesii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.17.1.3
2,3-trans-3,4-cis-leucocyanidin + NADPH
Pseudotsuga menziesii
proanthocyanidin biosynthesis
(2R,3S)-catechin + NADP+ + H2O
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.219
Pseudotsuga menziesii
-
douglas fir
-
1.17.1.3
Pseudotsuga menziesii
-
douglas fir
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.219
callus
-
Pseudotsuga menziesii
-
1.1.1.219
cell suspension culture
-
Pseudotsuga menziesii
-
1.17.1.3
cell suspension culture
-
Pseudotsuga menziesii
-
1.17.1.3
needle
-
Pseudotsuga menziesii
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.219
(+)-dihydroquercetin + NADPH
-
347999
Pseudotsuga menziesii
cis-3,4-leucocyanidin + NADP+
i.e. 5,7,3',4'-tetrahydroxyflavan-3,4-cis-diol, 2,3-trans-configuration retained
347999
Pseudotsuga menziesii
-
1.17.1.3
2,3-trans-3,4-cis-leucocyanidin + NADPH
enzyme acts mainly on the 3,4-cis rather than the 3,4-trans isomer
347999
Pseudotsuga menziesii
(2R,3S)-catechin + NADP+ + H2O
-
347999
Pseudotsuga menziesii
?
1.17.1.3
2,3-trans-3,4-cis-leucocyanidin + NADPH
proanthocyanidin biosynthesis
347999
Pseudotsuga menziesii
(2R,3S)-catechin + NADP+ + H2O
-
-
-
-
1.17.1.3
dihydroquercetin + NADPH
double step reduction
347999
Pseudotsuga menziesii
(2R,3S)-catechin + NADP+ + H2O
-
347999
Pseudotsuga menziesii
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.17.1.3
7.4
-
-
Pseudotsuga menziesii
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.219
6.4
8
no significant variations in activity
Pseudotsuga menziesii
1.17.1.3
6.4
8
no significant changes between pH 6.4 and pH 8.0
Pseudotsuga menziesii
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.219
NADPH
-
Pseudotsuga menziesii
1.17.1.3
NADPH
dependent
Pseudotsuga menziesii
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.219
NADPH
-
Pseudotsuga menziesii
1.17.1.3
NADPH
dependent
Pseudotsuga menziesii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.219
0.037
-
(+)-dihydroquercetin
estimation
Pseudotsuga menziesii
1.17.1.3
0.037
-
dihydroquercetin
pH 7.4
Pseudotsuga menziesii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.17.1.3
2,3-trans-3,4-cis-leucocyanidin + NADPH
Pseudotsuga menziesii
proanthocyanidin biosynthesis
(2R,3S)-catechin + NADP+ + H2O
-
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.219
callus
-
Pseudotsuga menziesii
-
1.1.1.219
cell suspension culture
-
Pseudotsuga menziesii
-
1.17.1.3
cell suspension culture
-
Pseudotsuga menziesii
-
1.17.1.3
needle
-
Pseudotsuga menziesii
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.219
(+)-dihydroquercetin + NADPH
-
347999
Pseudotsuga menziesii
cis-3,4-leucocyanidin + NADP+
i.e. 5,7,3',4'-tetrahydroxyflavan-3,4-cis-diol, 2,3-trans-configuration retained
347999
Pseudotsuga menziesii
-
1.17.1.3
2,3-trans-3,4-cis-leucocyanidin + NADPH
enzyme acts mainly on the 3,4-cis rather than the 3,4-trans isomer
347999
Pseudotsuga menziesii
(2R,3S)-catechin + NADP+ + H2O
-
347999
Pseudotsuga menziesii
?
1.17.1.3
2,3-trans-3,4-cis-leucocyanidin + NADPH
proanthocyanidin biosynthesis
347999
Pseudotsuga menziesii
(2R,3S)-catechin + NADP+ + H2O
-
-
-
-
1.17.1.3
dihydroquercetin + NADPH
double step reduction
347999
Pseudotsuga menziesii
(2R,3S)-catechin + NADP+ + H2O
-
347999
Pseudotsuga menziesii
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.17.1.3
7.4
-
-
Pseudotsuga menziesii
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.219
6.4
8
no significant variations in activity
Pseudotsuga menziesii
1.17.1.3
6.4
8
no significant changes between pH 6.4 and pH 8.0
Pseudotsuga menziesii