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Literature summary extracted from

  • Pugh, M.E.; Skibo, E.B.
    Inosine monophosphate dehydrogenase from porcine (Sus scrofa domestica) thymus: Purification and properties (1993), Comp. Biochem. Physiol. B, 2, 381-387.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.205 6-Chloro-9-beta-D-ribofuranosylpurine 5 '-phosphate acts at the IMP binding site Sus scrofa
1.1.1.205 AMP
-
 Sus scrofa
1.1.1.205 GMP
-
 Sus scrofa
1.1.1.205 NAD+ 44% inhibition at 1.5 M Sus scrofa
1.1.1.205 NH4+ 25% inhibition at 2.12 M Sus scrofa
1.1.1.205 xanthosine 5'-phosphate
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.205 0.007
-
 IMP
-
 Sus scrofa
1.1.1.205 0.016
-
 NAD+
-
 Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.205 K+
-
 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.205 57000
-
 SDS-PAGE Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.205 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.205 homogeneity Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.205 0.57
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
1.1.1.205 -20, no significant loss of activity for serveral weeks after ammonium sulfate precipitation, no loss of activity after Sephacryl S400 chromatography Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.205 IMP + NAD+ + H2O
-
 Sus scrofa XMP + NADH + H+
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.205 8
-
-
 Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.205 7.5 9 pH 7.5: about 50% of activity maximum, pH 9: about 50% of activity maximum Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.205 0.05
-
 GMP
-
 Sus scrofa
1.1.1.205 0.085
-
 xanthosine 5'-phosphate
-
 Sus scrofa
1.1.1.205 0.282
-
 AMP
-
 Sus scrofa