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Literature summary extracted from

  • Tokumoto, H.; Watanabe, K.; Fukushima, D.; Shimizu, T.; Hayaishi, O.
    An NADP-linked 15-hydroxyprostaglandin dehydrogenase specific for prostaglandin D2 from swine brain (1982), J. Biol. Chem., 257, 13576-13580.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.196 unstable Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.196 11,14-Eicosadienoic acid
-
 Sus scrofa
1.1.1.196 3,3',5-Triiodothyroacetic acid
-
 Sus scrofa
1.1.1.196 arachidonic acid
-
 Sus scrofa
1.1.1.196 Flufenamic acid
-
 Sus scrofa
1.1.1.196 indomethacin
-
 Sus scrofa
1.1.1.196 L-thyroxine
-
 Sus scrofa
1.1.1.196 linoleic acid
-
 Sus scrofa
1.1.1.196 N-ethylmaleimide
-
 Sus scrofa
1.1.1.196 p-chloromercuribenzoate
-
 Sus scrofa
1.1.1.196 palmitic acid
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.196 0.0004
-
 NADP+
-
 Sus scrofa
1.1.1.196 0.052
-
 Prostaglandin D2
-
 Sus scrofa
1.1.1.196 0.278
-
 prostaglandin D1
-
 Sus scrofa
1.1.1.196 0.35
-
 prostaglandin E2
-
 Sus scrofa
1.1.1.196 0.4
-
 prostaglandin F2alpha
-
 Sus scrofa
1.1.1.196 0.54
-
 prostaglandin I2
-
 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.196 28000
-
 gel electrophoresis Sus scrofa
1.1.1.196 29000
-
 1 * 29000 Sus scrofa
1.1.1.196 30000
-
 sedimentation equilibrium Sus scrofa
1.1.1.196 32000
-
 gel filtration Sus scrofa
1.1.1.196 52000
-
 gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.196 prostaglandin D2 + NADP+ Homo sapiens specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets 15-ketoprostaglandin D2 + NADPH + H+
-
 ?
1.1.1.196 prostaglandin D2 + NADP+ Sus scrofa specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets 15-ketoprostaglandin D2 + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.196 Homo sapiens
-
 human
-
1.1.1.196 Sus scrofa
-
 swine
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.196
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.196 blood platelet Homo sapiens
-
1.1.1.196 brain
-
 Homo sapiens
-
1.1.1.196 brain
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.196 0.0212
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
1.1.1.196 4°C, at pH 7 for 6 h, 54% loss of enzyme activity Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.196 prostaglandin D1 + NADP+
-
 Sus scrofa 15-ketoprostaglandin D1 + NADPH + H+
-
 ?
1.1.1.196 prostaglandin D2 + NADP+
-
 Homo sapiens 15-ketoprostaglandin D2 + NADPH + H+
-
 ?
1.1.1.196 prostaglandin D2 + NADP+
-
 Sus scrofa 15-ketoprostaglandin D2 + NADPH + H+
-
 ?
1.1.1.196 prostaglandin D2 + NADP+ specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets Homo sapiens 15-ketoprostaglandin D2 + NADPH + H+
-
 ?
1.1.1.196 prostaglandin D2 + NADP+ specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets Sus scrofa 15-ketoprostaglandin D2 + NADPH + H+
-
 ?
1.1.1.196 prostaglandin E2 + NADP+
-
 Sus scrofa 15-ketoprostaglandin E2 + NADPH + H+
-
 ?
1.1.1.196 prostaglandin F2alpha + NADP+
-
 Sus scrofa 15-ketoprostaglandin F2alpha + NADPH + H+
-
 ?
1.1.1.196 prostaglandin I2 + NADP+
-
 Sus scrofa 15-ketoprostaglandin I2 + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.196 monomer 1 * 28000, SDS-PAGE Sus scrofa
1.1.1.196 monomer 1 * 29000 Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.196 9.5
-
 optimal for reaction with prostaglandins D1, D2, E2 and I2 Sus scrofa
1.1.1.196 9.5 10 with prostaglandin F2alpha as substrate Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.196 7
-
 most stable at pH 7 with 54% loss of activity, loses 100% activity at pH 5, 70% at pH 6, 76% at pH 8 and 100% at pH 9 Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.196 NADP+ strict specificity for Sus scrofa