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Literature summary extracted from

  • Usui, S.; Hara, A.; Nakayama, T.; Sawada, H.
    Purification and characterization of two forms of microsomal carbonyl reductase in guinea pig liver (1984), Biochem. J., 223, 697-705.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.184 21-Hydroxy-5alpha-pregnane-3,20-dione
-
 Cavia porcellus
1.1.1.184 3,3-Tetramethyleneglutaric acid enzyme form CR2 inhibited, CR1 slightly Cavia porcellus
1.1.1.184 5beta-androstane-3alpha,17beta-diol
-
 Cavia porcellus
1.1.1.184 5beta-pregnane-3,20-dione
-
 Cavia porcellus
1.1.1.184 AgNO3 enzyme form CR1 inhibited, CR2 not Cavia porcellus
1.1.1.184 Ammonium molybdate enzyme form CR1 inhibited, CR2 not Cavia porcellus
1.1.1.184 androsterone CR2 inhibited, CR1 not Cavia porcellus
1.1.1.184 chlorpromazine
-
 Cavia porcellus
1.1.1.184 dicoumarol enzyme form CR2 inhibited, CR1 not Cavia porcellus
1.1.1.184 Hg2+
-
 Cavia porcellus
1.1.1.184 indomethacin
-
 Cavia porcellus
1.1.1.184 p-chloromercuribenzoate
-
 Cavia porcellus
1.1.1.184 progesterone
-
 Cavia porcellus
1.1.1.184 quercitrin
-
 Cavia porcellus
1.1.1.184 Sodium phenobarbitone enzyme form CR2 inhibited, CR1 slightly Cavia porcellus
1.1.1.184 testosterone
-
 Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.184 additional information
-
 additional information overview Cavia porcellus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.184 32000
-
 4 * 32000, enzyme form CR2, SDS-PAGE Cavia porcellus
1.1.1.184 34000
-
 gel filtration, enzyme form CR1 Cavia porcellus
1.1.1.184 37500
-
 1 * 37500, enzyme form CR1, SDS-PAGE Cavia porcellus
1.1.1.184 115000
-
 gel filtration, enzyme form CR2 Cavia porcellus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.184 Cavia porcellus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.184 2 enzyme forms: major form CR2, minor form CR1 Cavia porcellus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.184 liver
-
 Cavia porcellus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.184 additional information
-
-
 Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.184 2-nitrobenzaldehyde + NADPH
-
 Cavia porcellus 2-nitrobenzyl alcohol + NADP+
-
 ?
1.1.1.184 3-nitroacetophenone + NADPH
-
 Cavia porcellus ?
-
 ?
1.1.1.184 3-nitrobenzaldehyde + NADPH
-
 Cavia porcellus 3-nitrobenzyl alcohol + NADP+
-
 ?
1.1.1.184 4-nitroacetophenone + NADPH
-
 Cavia porcellus ?
-
 ?
1.1.1.184 4-nitrobenzaldehyde + NADPH
-
 Cavia porcellus 4-nitrobenzyl alcohol + NADP+
-
 ?
1.1.1.184 benzaldehyde + NADPH
-
 Cavia porcellus benzyl alcohol + NADP+
-
 ?
1.1.1.184 diacetyl + NADPH
-
 Cavia porcellus ?
-
 ?
1.1.1.184 additional information overview Cavia porcellus ?
-
 ?
1.1.1.184 additional information specificity of various enzyme forms Cavia porcellus ?
-
 ?
1.1.1.184 phenylglyoxal + NADPH
-
 Cavia porcellus ?
-
 ?
1.1.1.184 pyridine-4-aldehyde + NADPH
-
 Cavia porcellus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.184 monomer 1 * 37500, enzyme form CR1, SDS-PAGE Cavia porcellus
1.1.1.184 tetramer 4 * 32000, enzyme form CR2, SDS-PAGE Cavia porcellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.184 4.8 5.3 4-nitroacetophenone, pyridine 4-aldehyde, 5beta-dihydrotestosterone, enzyme CR2 Cavia porcellus
1.1.1.184 5.8 6.2 4-nitroacetophenone, pyridine 4-aldehyde, enzyme form CR1 Cavia porcellus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.184 NADP+
-
 Cavia porcellus
1.1.1.184 NADPH
-
 Cavia porcellus