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Literature summary extracted from

  • Nakayama, T.; Hara, A.; Sawada, H.
    Purification and characterization of a novel pyrazole-sensitive carbonyl reductase in guinea pig lung (1982), Arch. Biochem. Biophys., 217, 564-573.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.184 glycerol, 20% v/v, stabilizes Cavia porcellus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.184 dithiothreitol
-
 Cavia porcellus
1.1.1.184 FeSO4
-
 Cavia porcellus
1.1.1.184 N-ethylmaleimide
-
 Cavia porcellus
1.1.1.184 NAD+
-
 Cavia porcellus
1.1.1.184 NADP+
-
 Cavia porcellus
1.1.1.184 nicotinic acid weak Cavia porcellus
1.1.1.184 p-hydroxymercuribenzoate
-
 Cavia porcellus
1.1.1.184 phenylpyruvic acid
-
 Cavia porcellus
1.1.1.184 pyrazole
-
 Cavia porcellus
1.1.1.184 quercetin
-
 Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.184 additional information
-
 additional information
-
 Cavia porcellus
1.1.1.184 0.002
-
 pyridine 4-aldehyde cofactor NADPH Cavia porcellus
1.1.1.184 0.004
-
 4-Nitroacetophenone cofactor NADPH Cavia porcellus
1.1.1.184 0.009
-
 menadione cofactor NADPH Cavia porcellus
1.1.1.184 0.012
-
 methyl-1,4-benzoquinone cofactor NADPH Cavia porcellus
1.1.1.184 0.015
-
 pyridine 3-aldehyde cofactor NADPH Cavia porcellus
1.1.1.184 0.031
-
 Benzalacetone cofactor NADPH Cavia porcellus
1.1.1.184 0.039
-
 acetaldehyde cofactor NADPH Cavia porcellus
1.1.1.184 0.057
-
 methyl-1,4-benzoquinone cofactor NADH Cavia porcellus
1.1.1.184 0.091
-
 acetone cofactor NADPH Cavia porcellus
1.1.1.184 0.12
-
 crotonaldehyde cofactor NADPH Cavia porcellus
1.1.1.184 0.13
-
 pyridine 3-aldehyde cosubstrate NADH Cavia porcellus
1.1.1.184 0.24
-
 4-benzoylpyridine cofactor NADPH Cavia porcellus
1.1.1.184 0.33
-
 Benzalacetone cofactor NADH Cavia porcellus
1.1.1.184 0.5
-
 Pyridine-4-aldehyde cofactor NADH Cavia porcellus
1.1.1.184 3.1
-
 acetone cofactor NADH Cavia porcellus
1.1.1.184 3.3
-
 4-benzoylpyridine cofactor NADH Cavia porcellus
1.1.1.184 5.1
-
 acetaldehyde cofactor NADH Cavia porcellus
1.1.1.184 250
-
 crotonaldehyde cofactor NADH Cavia porcellus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.184 23000
-
 x * 23000, SDS-PAGE Cavia porcellus
1.1.1.184 86000
-
 gel filtration Cavia porcellus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.184 Cavia porcellus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.184 2 enzyme species Cavia porcellus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.184 lung
-
 Cavia porcellus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.184 additional information
-
-
 Cavia porcellus

Storage Stability

EC Number Storage Stability Organism
1.1.1.184 -20°C or 4°C, 20% v/v, glycerol, stable for 1 month Cavia porcellus
1.1.1.184 4°C, 0.02 mg/ml protein concentration, 0.15 M KCl, stable for at least several days, 90% loss of activity after 2 weeks Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.184 4-benzoylpyridine + NADPH
-
 Cavia porcellus ?
-
 ?
1.1.1.184 4-nitroacetophenone + NADPH
-
 Cavia porcellus ?
-
 ?
1.1.1.184 acetaldehyde + NADPH + H+
-
 Cavia porcellus ethanol + NADP+
-
 ?
1.1.1.184 acetone + NADPH
-
 Cavia porcellus propan-2-ol + NADP+
-
 ?
1.1.1.184 benzalacetone + NADPH
-
 Cavia porcellus ?
-
 ?
1.1.1.184 crotonaldehyde + NADPH
-
 Cavia porcellus 2-buten-1-ol + NADP+
-
 ?
1.1.1.184 menadione + NADPH
-
 Cavia porcellus ?
-
 ?
1.1.1.184 methyl-1,4-benzoquinone + NADPH
-
 Cavia porcellus ?
-
 ?
1.1.1.184 additional information overview Cavia porcellus ?
-
 ?
1.1.1.184 additional information NADH also acts as cofactor Cavia porcellus ?
-
 ?
1.1.1.184 additional information low reverse direction only detected with cyclohexanol Cavia porcellus ?
-
 ?
1.1.1.184 pyridine-3-aldehyde + NADPH
-
 Cavia porcellus ?
-
 ?
1.1.1.184 pyridine-4-aldehyde + NADPH
-
 Cavia porcellus ?
-
 ir

Subunits

EC Number Subunits Comment Organism
1.1.1.184 ? x * 23000, SDS-PAGE Cavia porcellus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.184 25
-
 pH 6-11, 15 min, stable Cavia porcellus
1.1.1.184 50
-
 pH 6-11, 15 min, about 20% loss of activity Cavia porcellus
1.1.1.184 60
-
 rapid inactivation above Cavia porcellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.184 4.8
-
 NADPH + 4-nitroacetophenone Cavia porcellus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.184 4.4
-
 unstable Cavia porcellus
1.1.1.184 6 11 25°C, 15 min, stable Cavia porcellus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.184 NADH
-
 Cavia porcellus
1.1.1.184 NADPH
-
 Cavia porcellus