BRENDA - Enzyme Database

Purification and properties of an NADPH-dependent carbonyl reductase from human brain. Relationship to prostaglandin 9-ketoreductase and xenobiotic ketone reductase

Wermuth, B.; J. Biol. Chem. 256, 1206-1213 (1981)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.184
4-Oxo-4H-benzopyran-2-carboxylic acid
-
Homo sapiens
1.1.1.184
chlorogenic acid
-
Homo sapiens
1.1.1.184
chlorpromazine
-
Homo sapiens
1.1.1.184
Cibacron blue
3G-A
Homo sapiens
1.1.1.184
D-catechin
-
Homo sapiens
1.1.1.184
dicoumarol
-
Homo sapiens
1.1.1.184
Ethacrynic acid
-
Homo sapiens
1.1.1.184
FAD
-
Homo sapiens
1.1.1.184
flavonoids
-
Homo sapiens
1.1.1.184
indomethacin
-
Homo sapiens
1.1.1.184
iodoacetate
-
Homo sapiens
1.1.1.184
NADP+
-
Homo sapiens
1.1.1.184
p-hydroxymercuribenzoate
-
Homo sapiens
1.1.1.184
quercetin
-
Homo sapiens
1.1.1.184
Retin
-
Homo sapiens
1.1.1.184
Tetramethyleneglutaric acid
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.184
additional information
-
additional information
overview
Homo sapiens
1.1.1.184
0.004
0.01
NADPH
+ 4-nitrobenzaldehyde, Km depending on enzyme form
Homo sapiens
1.1.1.184
0.015
0.017
ubiquinone-1
Km depending on enzyme form
Homo sapiens
1.1.1.184
0.045
0.06
menadione
Km depending on enzyme form
Homo sapiens
1.1.1.184
0.13
-
daunorubicin
enzyme form CR8
Homo sapiens
1.1.1.184
0.45
-
prostaglandin E1
enzyme form CR8
Homo sapiens
1.1.1.184
1.6
2
4-nitrobenzaldehyde
Km depending on enzyme form
Homo sapiens
1.1.1.184
4
5
Phenylglyoxal
Km depending on enzyme form
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.184
Iron
detected in enzyme by atomic absorption spectroscopy
Homo sapiens
1.1.1.184
Magnesium
detected in enzyme by atomic absorption spectroscopy
Homo sapiens
1.1.1.184
Zinc
detected in enzyme by atomic absorption spectroscopy
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.184
30000
-
gel filtration, SDS-PAGE, amino acid analysis
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.184
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.184
3 molecular forms: CR7, CR8, CR8.5
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.184
brain
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.184
8.3
-
-
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.184
2 ferricyanide + NADPH
-
347749
Homo sapiens
2 ferrocyanide + NADP+ + H+
-
-
-
?
1.1.1.184
2,3-bornanedione + NADPH
-
347749
Homo sapiens
?
-
-
-
?
1.1.1.184
4-carboxybenzaldehyde + NADPH
-
347749
Homo sapiens
4-carboxybenzyl alcohol + NADP+
-
-
-
?
1.1.1.184
4-nitrobenzaldehyde + NADPH
-
347749
Homo sapiens
4-nitrobenzyl alcohol + NADP+
-
-
-
?
1.1.1.184
9,10-phenanthrenequinone + NADPH
-
347749
Homo sapiens
?
-
-
-
?
1.1.1.184
alpha-tocopherolquinone + NADPH
-
347749
Homo sapiens
?
-
-
-
?
1.1.1.184
daunorubicin + NADPH
-
347749
Homo sapiens
?
-
-
-
?
1.1.1.184
duroquinone + NADPH
-
347749
Homo sapiens
?
-
-
-
?
1.1.1.184
hydrindantin + NADPH
-
347749
Homo sapiens
?
-
-
-
?
1.1.1.184
indole 3-acetaldehyde + NADPH
-
347749
Homo sapiens
indole-3-ol + NADP+
-
-
-
?
1.1.1.184
menadione + NADPH
-
347749
Homo sapiens
?
-
-
-
?
1.1.1.184
methylglyoxal + NADPH
-
347749
Homo sapiens
?
-
-
-
?
1.1.1.184
additional information
overview
347749
Homo sapiens
?
-
-
-
-
1.1.1.184
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
-
347749
Homo sapiens
NADP+ + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.1.184
phenylglyoxal + NADPH
-
347749
Homo sapiens
?
-
-
-
?
1.1.1.184
prostaglandin A1-GSH + NADPH
-
347749
Homo sapiens
?
-
-
-
?
1.1.1.184
ubiquinone-1 + NADPH
-
347749
Homo sapiens
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.184
monomer
1 * 30000, SDS-PAGE
Homo sapiens
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.184
25
-
assay at
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.184
6
-
menadione, 4-nitrobenzaldehyde, daunorubicin, in sodium phosphate buffer
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.184
NADPH
pro-4S hydrogen atom of the nicotinamide ring of NADPH is transferred to the substrate
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.184
NADPH
pro-4S hydrogen atom of the nicotinamide ring of NADPH is transferred to the substrate
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.184
4-Oxo-4H-benzopyran-2-carboxylic acid
-
Homo sapiens
1.1.1.184
chlorogenic acid
-
Homo sapiens
1.1.1.184
chlorpromazine
-
Homo sapiens
1.1.1.184
Cibacron blue
3G-A
Homo sapiens
1.1.1.184
D-catechin
-
Homo sapiens
1.1.1.184
dicoumarol
-
Homo sapiens
1.1.1.184
Ethacrynic acid
-
Homo sapiens
1.1.1.184
FAD
-
Homo sapiens
1.1.1.184
flavonoids
-
Homo sapiens
1.1.1.184
indomethacin
-
Homo sapiens
1.1.1.184
iodoacetate
-
Homo sapiens
1.1.1.184
NADP+
-
Homo sapiens
1.1.1.184
p-hydroxymercuribenzoate
-
Homo sapiens
1.1.1.184
quercetin
-
Homo sapiens
1.1.1.184
Retin
-
Homo sapiens
1.1.1.184
Tetramethyleneglutaric acid
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.184
additional information
-
additional information
overview
Homo sapiens
1.1.1.184
0.004
0.01
NADPH
+ 4-nitrobenzaldehyde, Km depending on enzyme form
Homo sapiens
1.1.1.184
0.015
0.017
ubiquinone-1
Km depending on enzyme form
Homo sapiens
1.1.1.184
0.045
0.06
menadione
Km depending on enzyme form
Homo sapiens
1.1.1.184
0.13
-
daunorubicin
enzyme form CR8
Homo sapiens
1.1.1.184
0.45
-
prostaglandin E1
enzyme form CR8
Homo sapiens
1.1.1.184
1.6
2
4-nitrobenzaldehyde
Km depending on enzyme form
Homo sapiens
1.1.1.184
4
5
Phenylglyoxal
Km depending on enzyme form
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.184
Iron
detected in enzyme by atomic absorption spectroscopy
Homo sapiens
1.1.1.184
Magnesium
detected in enzyme by atomic absorption spectroscopy
Homo sapiens
1.1.1.184
Zinc
detected in enzyme by atomic absorption spectroscopy
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.184
30000
-
gel filtration, SDS-PAGE, amino acid analysis
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.184
3 molecular forms: CR7, CR8, CR8.5
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.184
brain
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.184
8.3
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.184
2 ferricyanide + NADPH
-
347749
Homo sapiens
2 ferrocyanide + NADP+ + H+
-
-
-
?
1.1.1.184
2,3-bornanedione + NADPH
-
347749
Homo sapiens
?
-
-
-
?
1.1.1.184
4-carboxybenzaldehyde + NADPH
-
347749
Homo sapiens
4-carboxybenzyl alcohol + NADP+
-
-
-
?
1.1.1.184
4-nitrobenzaldehyde + NADPH
-
347749
Homo sapiens
4-nitrobenzyl alcohol + NADP+
-
-
-
?
1.1.1.184
9,10-phenanthrenequinone + NADPH
-
347749
Homo sapiens
?
-
-
-
?
1.1.1.184
alpha-tocopherolquinone + NADPH
-
347749
Homo sapiens
?
-
-
-
?
1.1.1.184
daunorubicin + NADPH
-
347749
Homo sapiens
?
-
-
-
?
1.1.1.184
duroquinone + NADPH
-
347749
Homo sapiens
?
-
-
-
?
1.1.1.184
hydrindantin + NADPH
-
347749
Homo sapiens
?
-
-
-
?
1.1.1.184
indole 3-acetaldehyde + NADPH
-
347749
Homo sapiens
indole-3-ol + NADP+
-
-
-
?
1.1.1.184
menadione + NADPH
-
347749
Homo sapiens
?
-
-
-
?
1.1.1.184
methylglyoxal + NADPH
-
347749
Homo sapiens
?
-
-
-
?
1.1.1.184
additional information
overview
347749
Homo sapiens
?
-
-
-
-
1.1.1.184
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
-
347749
Homo sapiens
NADP+ + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.1.184
phenylglyoxal + NADPH
-
347749
Homo sapiens
?
-
-
-
?
1.1.1.184
prostaglandin A1-GSH + NADPH
-
347749
Homo sapiens
?
-
-
-
?
1.1.1.184
ubiquinone-1 + NADPH
-
347749
Homo sapiens
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.184
monomer
1 * 30000, SDS-PAGE
Homo sapiens
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.184
25
-
assay at
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.184
6
-
menadione, 4-nitrobenzaldehyde, daunorubicin, in sodium phosphate buffer
Homo sapiens