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Literature summary extracted from
- Purification and characterization of oxopantoyl lactone reductase from higher plants: role in pantothenate synthesis (1994), Bot. Acta, 107, 191-200.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.168 | 2-keto-4-hydroxybutyrolactone | - |
Spinacia oleracea |  |
| 1.1.1.168 | oxopyrrolidines | - |
Spinacia oleracea | |
| 1.1.1.168 | Reactive Red 3A | - |
Spinacia oleracea | |
| 1.1.1.214 | 2-keto-4-hydroxybutyrolactone | strong and uncompetitive inhibitor | Spinacia oleracea | |
| 1.1.1.214 | substituted 2,5-dioxopyrrolidines | strong and uncompetitive inhibitors | Spinacia oleracea | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.168 | 0.01 | - |
isatin | - |
Spinacia oleracea | |
| 1.1.1.168 | 0.012 | - |
bornanedione | - |
Spinacia oleracea | |
| 1.1.1.168 | 0.015 | - |
8-bromobornanedione | - |
Spinacia oleracea | |
| 1.1.1.168 | 0.026 | - |
oxopantoyl lactone | - |
Spinacia oleracea | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.1.214 | chloroplast | - |
Spinacia oleracea | 9507 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.168 | 33000 | 36000 | gel filtration, SDS-PAGE | Spinacia oleracea |
| 1.1.1.214 | 33000 | 36000 | - |
Spinacia oleracea |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.214 | ketopantoyl lactone + NADPH | Spinacia oleracea | - |
pantoyl lactone + NADP+ | the enzyme is involved in pantothenate biosynthesis, pantoyl lactone, the putative product of the reaction, together with beta-alanine and ATP are the substrate of pantothenate synthase | ? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.168 | Spinacia oleracea | - |
spinach | - |
| 1.1.1.214 | Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.168 | - |
Spinacia oleracea |
| 1.1.1.214 | using affinity chromatography on Red-Agarose and several subsequent ion exchange steps | Spinacia oleracea |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.214 | additional information | - |
- |
Spinacia oleracea |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.168 | 4°C, 1 year, 50% glycerol, 10% loss of activity | Spinacia oleracea |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.168 | 2-dehydropantoyl lactone + NADPH | - |
Spinacia oleracea | (R)-pantoyl lactone + NADP+ | - |
ir | |
| 1.1.1.168 | 8-bromobornanedione + NADPH | - |
Spinacia oleracea | ? | - |
? | |
| 1.1.1.168 | bornanedione + NADPH | - |
Spinacia oleracea | ? | - |
? | |
| 1.1.1.168 | isatin + NADPH | - |
Spinacia oleracea | ? | - |
? | |
| 1.1.1.168 | additional information | not: linear form of oxopantoate | Spinacia oleracea | additional information | - |
? | |
| 1.1.1.168 | oxopantoyl lactone + NADPH | - |
Spinacia oleracea | ? | - |
? | |
| 1.1.1.214 | acenaphthenequinone | - |
Spinacia oleracea | ? | - |
? | |
| 1.1.1.214 | bornanedione + NADPH | - |
Spinacia oleracea | ? | - |
? | |
| 1.1.1.214 | isatin + NADPH | - |
Spinacia oleracea | ? | - |
? | |
| 1.1.1.214 | ketopantoyl lactone + NADPH | - |
Spinacia oleracea | pantoyl lactone + NADP+ | - |
? | |
| 1.1.1.214 | ketopantoyl lactone + NADPH | - |
Spinacia oleracea | pantoyl lactone + NADP+ | the enzyme is involved in pantothenate biosynthesis, pantoyl lactone, the putative product of the reaction, together with beta-alanine and ATP are the substrate of pantothenate synthase | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.168 | monomer | 1* 36000, SDS-PAGE | Spinacia oleracea |
| 1.1.1.214 | monomer | 1 * 33000-36000 | Spinacia oleracea |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.168 | 56 | - |
0.35 M anhydrous sodium sulfate, complete inactivation | Spinacia oleracea |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.168 | 6.8 | - |
- |
Spinacia oleracea |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.168 | NADPH | strictly dependent on | Spinacia oleracea | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.168 | 0.0025 | - |
Reactive Red 3A | - |
Spinacia oleracea | |
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