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Literature summary extracted from

  • Hareland, W.A.; Crawford, R.L.; Chapman, P.J.; Dagley, S.
    Metabolic function and properties of 4-hydroxyphenylacetic acid 1-hydroxylase from Pseudomonas acidovorans (1975), J. Bacteriol., 121, 272-285.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.18 3,4-dihydroxyphenylacetic acid
-
 Delftia acidovorans
1.14.13.18 4-Hydroxy-3-methylphenylacetic acid
-
 Delftia acidovorans
1.14.13.18 4-hydroxyphenylpropionic acid
-
 Delftia acidovorans
1.14.13.18 DL-4-hydroxymandelic acid
-
 Delftia acidovorans
1.14.13.18 KCl
-
 Delftia acidovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.18 0.031
-
 4-hydroxyphenylacetate
-
 Delftia acidovorans
1.14.13.18 0.067
-
 O2
-
 Delftia acidovorans
1.14.13.18 0.095
-
 NADH
-
 Delftia acidovorans
1.14.13.18 0.25
-
 NADPH
-
 Delftia acidovorans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.18 Mg2+ required Delftia acidovorans

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.18 Delftia acidovorans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.18
-
 Delftia acidovorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.18 2.2
-
-
 Delftia acidovorans

Storage Stability

EC Number Storage Stability Organism
1.14.13.18 4°C, in the presence of dithioerythritol Delftia acidovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.18 4-hydroxy-2-methylphenylacetate + NAD(P)H + O2
-
 Delftia acidovorans ?
-
 ?
1.14.13.18 4-hydroxyhydratropate + NAD(P)H + O2
-
 Delftia acidovorans 2-methylhomogentisate + NAD(P)+ + H2O
-
 ?
1.14.13.18 4-hydroxyphenoxyacetate + ?
-
 Delftia acidovorans hydroquinone + glycolate + ?
-
 ?
1.14.13.18 4-hydroxyphenylacetate + NAD(P)H + O2
-
 Delftia acidovorans homogentisate + NAD(P)+ + H2O
-
 ?
1.14.13.18 4-hydroxyphenylacetate + NADH + H+ + O2
-
 Delftia acidovorans homogentisate + NAD+ + H2O
-
 r
1.14.13.18 additional information low activity with: 4-hydroxyphenylpropionate, 4-hydroxy-3-methylphenylacetate, 3,4-dihydroxyphenylacetate Delftia acidovorans ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.18 25
-
 assay at Delftia acidovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.18 7.3
-
-
 Delftia acidovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.18 FAD FAD required Delftia acidovorans
1.14.13.18 NADH same maximal velocity with NADH and NADPH Delftia acidovorans
1.14.13.18 NADPH same maximal velocity with NADH and NADPH Delftia acidovorans