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Literature summary extracted from

  • French, K.J.; Strickler, M.D.; Rock, D.A.; Rock, D.A.; Bennett, G.A.; Wahlstrom, J.L.; Goldstein, B.M.; Jones, J.P.
    Benign synthesis of 2-ethylhexanoic acid by cytochrome P450cam: enzymatic, crystallographic, and theoretical studies (2001), Biochemistry, 40, 9532-9538.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.15.1
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.1 0.0016
-
 (+)-Camphor
-
 Pseudomonas putida
1.14.15.1 0.068
-
 (S)-2-ethylhexanol
-
 Pseudomonas putida
1.14.15.1 0.086
-
 (R)-2-ethylhexanol
-
 Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.1 (+)-camphor + putidaredoxin + O2 Pseudomonas putida
-
 (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O i.e. (+)-exo-5-hydroxycamphor ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.1 Pseudomonas putida
-
 Accession Number PC00183
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.1 (+)-camphor + putidaredoxin + O2
-
 Pseudomonas putida (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O i.e. (+)-exo-5-hydroxycamphor ?
1.14.15.1 (R)-2-ethylhexanol + reduced putidaredoxin + O2
-
 Pseudomonas putida (R)-2-ethylhexanoic acid + oxidized putidaredoxin + H2O
-
 r
1.14.15.1 (R)-3-ethylhexanol + putidaredoxin + O2
-
 Pseudomonas putida 2-ethylhexanoic acid + 2-ethyl-1,2-hexanediol + 2-ethyl-1,3-hexanediol + 2-ethyl-1,4-hexanediol + oxidized putidaredoxin + H2O ratio: 50:13:15:8 ?
1.14.15.1 (S)-2-ethylhexanol + reduced putidaredoxin + O2
-
 Pseudomonas putida (S)-2-ethylhexanoic acid + oxidized putidaredoxin + H2O
-
 r
1.14.15.1 (S)-3-ethylhexanol + putidaredoxin + O2 the (S)-isomer is turned over 1.4times faster than the (R)-isomer Pseudomonas putida 2-ethylhexanoic aicd + 2-ethyl-1,2-hexanediol + 2-ethyl-1,3-hexanediol + 2-ethyl-1,4-hexanediol + oxidized putidaredoxin + H2O ratio: 15:53:28:10 ?