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Literature summary extracted from

  • Nickerson, D.P.; Wong, L.L.
    The dimerization of Pseudomonas putida cytochrome P450cam: practical consequences and engineering of a monomeric enzyme (1997), Protein Eng., 10, 1357-1361.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.1 C334A identical with the wild-type monomer in terms of optical spectra, camphor-binding and turnover Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.1 (+)-camphor + putidaredoxin + O2 Pseudomonas putida
-
 (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O i.e. (+)-exo-5-hydroxycamphor ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.1 Pseudomonas putida
-
 Accession Number PC00183
-

Storage Stability

EC Number Storage Stability Organism
1.14.15.1 4°C, monomeric cytochrome P450cam can be stored at low protein concentrations for several days without appreciable accumulation of the dimer Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.1 (+)-camphor + putidaredoxin + O2
-
 Pseudomonas putida (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O i.e. (+)-exo-5-hydroxycamphor ?

Subunits

EC Number Subunits Comment Organism
1.14.15.1 More cytochrome P450cam dimerizes via the formation of an intermolecular disulfide bond Pseudomonas putida