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Literature summary extracted from

  • Gelb, M.H.; Mälkönen, P.; Sligar, S.G.
    Cytochrome P450cam catalyzed epoxidation of dehydrocamphor (1982), Biochem. Biophys. Res. Commun., 104, 853-858.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.1 cytoplasm
-
 Pseudomonas putida 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.15.1 Fe2+ requirement, enzyme complex with two FeS-protein components Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.1 (+)-camphor + putidaredoxin + O2 Pseudomonas putida
-
 (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O i.e. (+)-exo-5-hydroxycamphor ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.1 Pseudomonas putida
-
 PpG786, mutant derived from PpG1
-
1.14.15.1 Pseudomonas putida
-
 Accession Number PC00183
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.1 (+)-camphor + putidaredoxin + O2
-
 Pseudomonas putida (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O i.e. (+)-exo-5-hydroxycamphor ?
1.14.15.1 1-dehydrocamphor + putidaredoxin + O2
-
 Pseudomonas putida exo-5,6-epoxycamphor + oxidized putidaredoxin + H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.1 20
-
 assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.1 7
-
 assay at Pseudomonas putida
1.14.15.1 7.4
-
 assay at Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.1 cytochrome m
-
 Pseudomonas putida
1.14.15.1 NADH
-
 Pseudomonas putida