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Literature summary extracted from

  • Nagasawa, T.; Ohkishi, H.; Yamada, H.
    3-Chloro-D-alanine chloride-lyase (deaminating) of Pseudomonas putida CR 1-1 (1984), Prog. Clin. Biol. Res., 144A, 365-375.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.5.1.2
-
 Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.5.1.2 AgNO3
-
 Pseudomonas putida
4.5.1.2 CuSO3
-
 Pseudomonas putida
4.5.1.2 cycloserine
-
 Pseudomonas putida
4.5.1.2 D-penicillamine
-
 Pseudomonas putida
4.5.1.2 HgCl2
-
 Pseudomonas putida
4.5.1.2 iodoacetate
-
 Pseudomonas putida
4.5.1.2 KCN
-
 Pseudomonas putida
4.5.1.2 phenylhydrazine
-
 Pseudomonas putida
4.5.1.2 thiol reagents
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.5.1.2 0.061
-
 3-chloro-D-alanine
-
 Pseudomonas putida
4.5.1.2 0.18
-
 D-cysteine
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.5.1.2 38000
-
 2 * 38000, SDS-PAGE Pseudomonas putida
4.5.1.2 76000
-
 sedimentation equilibrium Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.5.1.2 3-chloro-D-alanine + H2O Pseudomonas putida
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.5.1.2 Pseudomonas putida
-
 CR1-1, AKU 867
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.5.1.2 CR1-1 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.5.1.2 310
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.5.1.2 3-chloro-D-alanine + 2-mercaptoethanol
-
 Pseudomonas putida S-(2-hydroxyethyl)-D-cysteine
-
 ?
4.5.1.2 3-chloro-D-alanine + allyl mercaptane
-
 Pseudomonas putida S-allyl-D-cysteine
-
 ?
4.5.1.2 3-chloro-D-alanine + alpha-thiolglycerol
-
 Pseudomonas putida S-(2,3-dihydroxypropyl)-D-cysteine
-
 ?
4.5.1.2 3-chloro-D-alanine + ethyl-mercaptane
-
 Pseudomonas putida S-ethyl-D-cysteine
-
 ?
4.5.1.2 3-chloro-D-alanine + H2O
-
 Pseudomonas putida pyruvate + HCl + NH3
-
 ir
4.5.1.2 3-chloro-D-alanine + H2O
-
 Pseudomonas putida ?
-
 ?
4.5.1.2 3-chloro-D-alanine + methyl-mercaptane
-
 Pseudomonas putida S-methyl-D-cysteine
-
 ?
4.5.1.2 3-chloro-D-alanine + n-butyl-mercaptane
-
 Pseudomonas putida S-n-butyl-D-cysteine
-
 ?
4.5.1.2 3-chloro-D-alanine + n-propyl-mercaptane
-
 Pseudomonas putida S-n-propyl-D-cysteine
-
 ?
4.5.1.2 3-chloro-D-alanine + NaHS
-
 Pseudomonas putida D-cysteine + NaCl
-
 ir
4.5.1.2 3-chloro-D-alanine + phenyl-mercaptane
-
 Pseudomonas putida S-phenyl-D-cysteine
-
 ?

Subunits

EC Number Subunits Comment Organism
4.5.1.2 dimer 2 * 38000, SDS-PAGE Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5.1.2 9
-
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
4.5.1.2 pyridoxal 5'-phosphate
-
 Pseudomonas putida