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Literature summary extracted from

  • Yamada, H.; Nagasawa, T.; Ohkishi, H.; Kawakami, B.; Tani, Y.
    Synthesis of D-cysteine from 3-chloro-D-alanine and hydrogen sulfide by 3-chloro-D-alanine hydrogen chloride-lyase (deaminating) of Pseudomonas putida (1981), Biochem. Biophys. Res. Commun., 100, 1104-1110.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.5.1.2 8.1
-
 3-chloro-D-alanine
-
 Pseudomonas putida
4.5.1.2 46
-
 H2S
-
 Pseudomonas putida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.5.1.2 cytoplasm
-
 Pseudomonas putida 5737
-
4.5.1.2 soluble
-
 Pseudomonas putida
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.5.1.2 3-chloro-D-alanine + H2O Pseudomonas putida
-
 ?
-
 ?
4.5.1.2 3-chloro-D-alanine + H2O Pseudomonas putida CR1-1
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.5.1.2 Pseudomonas putida
-
 CR1-1
-
4.5.1.2 Pseudomonas putida CR1-1
-
 CR1-1
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.5.1.2 additional information
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.5.1.2 3-chloro-D-alanine + H2O
-
 Pseudomonas putida pyruvate + HCl + NH3
-
 ir
4.5.1.2 3-chloro-D-alanine + H2O
-
 Pseudomonas putida CR1-1 pyruvate + HCl + NH3
-
 ir
4.5.1.2 3-chloro-D-alanine + H2O
-
 Pseudomonas putida ?
-
 ?
4.5.1.2 3-chloro-D-alanine + H2O
-
 Pseudomonas putida CR1-1 ?
-
 ?
4.5.1.2 3-chloro-D-alanine + H2S
-
 Pseudomonas putida D-cysteine + HCl
-
 ir
4.5.1.2 3-chloro-D-alanine + H2S
-
 Pseudomonas putida CR1-1 D-cysteine + HCl
-
 ir
4.5.1.2 additional information specific for 3-chloro-D-alanine Pseudomonas putida ?
-
 ?
4.5.1.2 additional information specific for 3-chloro-D-alanine Pseudomonas putida CR1-1 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.5.1.2 40
-
-
 Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5.1.2 8.5
-
 cysteine synthesis Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.5.1.2 7 10
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
4.5.1.2 pyridoxal 5'-phosphate
-
 Pseudomonas putida