BRENDA - Enzyme Database

Characterization of the coenzyme-B12-dependent glutamate mutase from Clostridium cochlearium produced in Escherichia coli

Zelder, O.; Beatrix, B.; Leutbecher, U.; Buckel, W.; Eur. J. Biochem. 226, 577-585 (1994)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.4.99.1
overexpression of polypeptide chains sigma and epsilon in Escherichia coli
Clostridium cochlearium
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.99.1
2-Methyleneglutaric acid
-
Clostridium cochlearium
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.99.1
Glu
Clostridium cochlearium
first step in Glu fermentation pathway
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.99.1
Clostridium cochlearium
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.4.99.1
purification of component E and component S
Clostridium cochlearium
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.99.1
Glu
first step in Glu fermentation pathway
3463
Clostridium cochlearium
?
-
-
-
-
5.4.99.1
L-Glu
-
3463
Clostridium cochlearium
threo-3-Methylaspartate
-
3463
Clostridium cochlearium
-
Subunits
EC Number
Subunits
Commentary
Organism
5.4.99.1
?
component E is a dimer, epsilon2, with epsilon = MW 53500, + component S is a monomer, sigma = 14800
Clostridium cochlearium
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.4.99.1
Cobalamin
component S binds coenzyme B12; dependent on
Clostridium cochlearium
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.99.1
overexpression of polypeptide chains sigma and epsilon in Escherichia coli
Clostridium cochlearium
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.4.99.1
Cobalamin
component S binds coenzyme B12; dependent on
Clostridium cochlearium
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.99.1
2-Methyleneglutaric acid
-
Clostridium cochlearium
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.99.1
Glu
Clostridium cochlearium
first step in Glu fermentation pathway
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.99.1
purification of component E and component S
Clostridium cochlearium
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.99.1
Glu
first step in Glu fermentation pathway
3463
Clostridium cochlearium
?
-
-
-
-
5.4.99.1
L-Glu
-
3463
Clostridium cochlearium
threo-3-Methylaspartate
-
3463
Clostridium cochlearium
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.4.99.1
?
component E is a dimer, epsilon2, with epsilon = MW 53500, + component S is a monomer, sigma = 14800
Clostridium cochlearium