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Literature summary extracted from

  • Nock, L.P.; Mazelis, M.
    The C-S lyases of higher plants. Direct comparison of the physical properties of homogeneous alliin lyase of garlic (Allium sativum) and onion (Allium cepa) (1987), Plant Physiol., 85, 1079-1083.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.4 42000
-
 2 * 42000, gel filtration on a column equilibrated with 6 M urea Allium sativum
4.4.1.4 50000
-
 4 * 50000, gel filtration on a column equilibrated with 6 M urea and 0.1% SDS Allium cepa
4.4.1.4 85000
-
 gel filtration Allium sativum
4.4.1.4 200000
-
 gel filtration Allium cepa

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.4 Allium cepa
-
-
-
4.4.1.4 Allium sativum
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.4.1.4 side-chain modification glycoprotein Allium cepa
4.4.1.4 side-chain modification glycoprotein Allium sativum
4.4.1.4 side-chain modification contains 5.5% carbohydrate, 40% as pentose Allium sativum

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.4
-
 Allium sativum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.4 23.2
-
-
 Allium cepa
4.4.1.4 109
-
-
 Allium sativum

Subunits

EC Number Subunits Comment Organism
4.4.1.4 dimer 2 * 42000, gel filtration on a column equilibrated with 6 M urea Allium sativum
4.4.1.4 tetramer 4 * 50000, gel filtration on a column equilibrated with 6 M urea and 0.1% SDS Allium cepa

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.4 pyridoxal 5'-phosphate contains 1.68 mol of pyridoxal 5'-phosphate per mol of enzyme Allium sativum
4.4.1.4 pyridoxal 5'-phosphate contains 3.76 mol of pyridoxal 5'-phosphate per mol of enzyme Allium cepa
4.4.1.4 pyridoxal 5'-phosphate enzyme contains pyridoxal 5'-phosphate Allium cepa
4.4.1.4 pyridoxal 5'-phosphate enzyme contains pyridoxal 5'-phosphate Allium sativum