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Literature summary extracted from

  • Won, T.; Mazelis, M.
    The C-S lyases of higher plants. Purification and characterization of homogeneous alliin lyase of leek (Allium porrum) (1989), Physiol. Plant., 77, 87-92.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.4 Aminooxyacetate
-
 Allium ampeloprasum
4.4.1.4 hydroxylamine
-
 Allium ampeloprasum
4.4.1.4 NaCN
-
 Allium ampeloprasum
4.4.1.4 Trinitrobenzenesulfonic acid 2.5 mM, 50% inhibition Allium ampeloprasum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.4 10
-
 S-allyl-L-Cys sulfoxide
-
 Allium ampeloprasum
4.4.1.4 13
-
 S-ethyl-L-Cys sulfoxide
-
 Allium ampeloprasum
4.4.1.4 55
-
 S-methyl-L-Cys sulfoxide
-
 Allium ampeloprasum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.4 48000
-
 x * 48000, SDS-PAGE Allium ampeloprasum
4.4.1.4 386000
-
 2 stable enzyme forms detected: MW 386000 Da and 580000 Da, gel filtration Allium ampeloprasum
4.4.1.4 580000
-
 2 stable enzyme forms detected: MW 386000 Da and 580000 Da, gel filtration Allium ampeloprasum

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.4 Allium ampeloprasum
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.4.1.4 side-chain modification glycoprotein Allium ampeloprasum
4.4.1.4 side-chain modification major sugars are mannose, glucose and galactose Allium ampeloprasum

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.4
-
 Allium ampeloprasum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.4 2 S-ethyl-L-Cys sulfoxide + H2O
-
 Allium ampeloprasum ethyl ethanethiosulfinate + 2 pyruvate + 2 NH3
-
 ?
4.4.1.4 S-allyl-L-cysteine sulfoxide + H2O
-
 Allium ampeloprasum 2-propene-thioic acid + pyruvate + NH4+ + H+
-
 ?
4.4.1.4 S-methyl-L-Cys sulfoxide 40% of the activity with S-ethyl-L-Cys sulfoxide Allium ampeloprasum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.4 ? x * 48000, SDS-PAGE Allium ampeloprasum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.4 8
-
-
 Allium ampeloprasum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.4.1.4 2.5
-
 Trinitrobenzenesulfonic acid
-
 Allium ampeloprasum