BRENDA - Enzyme Database

Adenosylcobalamin-dependent glutamate mutase from Clostridium tetanomorphum. Overexpression in Escherichia coli, purification, and characterization of the recombinant enzyme

Holloway, D.E.; Marsh, E.N.G.; J. Biol. Chem. 269, 20425-20430 (1994)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.4.99.1
overexpression of component E and S in Escherichia coli
Clostridium tetanomorphum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.99.1
Clostridium tetanomorphum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.4.99.1
cloning and overexpression in Escherichia coli allows component E to be obtained in homogeneous form, free of inhibiting cobamides and traces of component S
Clostridium tetanomorphum
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.4.99.1
additional information
-
component E
Clostridium tetanomorphum
Storage Stability
EC Number
Storage Stability
Organism
5.4.99.1
-20°C, component E in presence of 50% glycerol, stable for several months
Clostridium tetanomorphum
5.4.99.1
4°C or -20°C, component S in concentrated solution in the absence of thiols, stable for several months
Clostridium tetanomorphum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.99.1
L-Glu
-
3460
Clostridium tetanomorphum
threo-3-Methylaspartate
-
3460
Clostridium tetanomorphum
-
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.99.1
overexpression of component E and S in Escherichia coli
Clostridium tetanomorphum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.99.1
cloning and overexpression in Escherichia coli allows component E to be obtained in homogeneous form, free of inhibiting cobamides and traces of component S
Clostridium tetanomorphum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.4.99.1
additional information
-
component E
Clostridium tetanomorphum
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
5.4.99.1
-20°C, component E in presence of 50% glycerol, stable for several months
Clostridium tetanomorphum
5.4.99.1
4°C or -20°C, component S in concentrated solution in the absence of thiols, stable for several months
Clostridium tetanomorphum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.99.1
L-Glu
-
3460
Clostridium tetanomorphum
threo-3-Methylaspartate
-
3460
Clostridium tetanomorphum
-