BRENDA - Enzyme Database

Cloning and characterization of a soluble kynurenine aminotransferase from rat brain: identity with kidney cysteine conjugate beta-lyase

Alberati-Giani, D.; Malherbe, P.; Köhler, C.; Lang, G.; Kiefer, V.; Lahm, H.W.; Cesura, A.M.; J. Neurochem. 64, 1448-1455 (1995)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.6.1.7
transient expression in HEK-293 cells
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.7
0.083
-
L-kynurenine
pH 8.0, 37°C, recombinant enzyme, cosubstrate 2-oxoglutarate
Rattus norvegicus
2.6.1.7
1.2
-
L-kynurenine
pH 8.0, 37°C, recombinant enzyme, cosubstrate pyruvate
Rattus norvegicus
2.6.1.7
1.7
-
L-kynurenine
pH 8.0, 37°C, cosubstrate pyruvate
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.7
cytosol
-
Rattus norvegicus
5829
-
2.6.1.7
soluble
-
Rattus norvegicus
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.7
47000
-
2 * 47000, SDS-PAGE
Rattus norvegicus
2.6.1.7
90000
100000
gel filtration
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.7
Rattus norvegicus
-
identical to kidney soluble cysteine conjugate beta-lyase also referred to as glutamine transaminase K, K stands for kidney
-
4.4.1.13
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.7
DEAE-Sepharose, phenyl-Sepharose, Superose 12, Mono Q
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.7
brain
-
Rattus norvegicus
-
2.6.1.7
kidney
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.7
0.36
-
-
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
L-kynurenine + 2-oxoglutarate
soluble kidney/brain kynurenine aminotransferase has both amino transferase and cysteine conjugate beta-lyase, i.e., glutamine transaminase K activities
34561
Rattus norvegicus
kynurenic acid + L-glutamate + H2O
-
34561
Rattus norvegicus
?
2.6.1.7
L-kynurenine + pyruvate
100fold higher maximal velocity than with 2-oxoglutarate
34561
Rattus norvegicus
kynurenic acid + L-alanine + H2O
-
34561
Rattus norvegicus
?
Subunits
EC Number
Subunits
Commentary
Organism
2.6.1.7
dimer
2 * 47000, SDS-PAGE
Rattus norvegicus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.7
transient expression in HEK-293 cells
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.7
0.083
-
L-kynurenine
pH 8.0, 37°C, recombinant enzyme, cosubstrate 2-oxoglutarate
Rattus norvegicus
2.6.1.7
1.2
-
L-kynurenine
pH 8.0, 37°C, recombinant enzyme, cosubstrate pyruvate
Rattus norvegicus
2.6.1.7
1.7
-
L-kynurenine
pH 8.0, 37°C, cosubstrate pyruvate
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.7
cytosol
-
Rattus norvegicus
5829
-
2.6.1.7
soluble
-
Rattus norvegicus
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.7
47000
-
2 * 47000, SDS-PAGE
Rattus norvegicus
2.6.1.7
90000
100000
gel filtration
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.7
DEAE-Sepharose, phenyl-Sepharose, Superose 12, Mono Q
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.7
brain
-
Rattus norvegicus
-
2.6.1.7
kidney
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.7
0.36
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
L-kynurenine + 2-oxoglutarate
soluble kidney/brain kynurenine aminotransferase has both amino transferase and cysteine conjugate beta-lyase, i.e., glutamine transaminase K activities
34561
Rattus norvegicus
kynurenic acid + L-glutamate + H2O
-
34561
Rattus norvegicus
?
2.6.1.7
L-kynurenine + pyruvate
100fold higher maximal velocity than with 2-oxoglutarate
34561
Rattus norvegicus
kynurenic acid + L-alanine + H2O
-
34561
Rattus norvegicus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.6.1.7
dimer
2 * 47000, SDS-PAGE
Rattus norvegicus