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Literature summary extracted from

  • Yamauchi, A.; Stijntjes, G.J.; Commandeur, J.N.M.; Vermeulen, N.P.E
    Purification of glutamine transaminase K/cysteine conjugate beta-lyase from rat renal cytosol based on hydrophobic interaction HPLC and gel permeation FPLC (1993), Protein Expr. Purif., 4, 552-562.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.13 0.33
-
 S-1,2-dichlorovinyl-L-cysteine
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.4.1.13 cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.13 47400
-
 2 * 47400, SDS-PAGE Rattus norvegicus
4.4.1.13 100000
-
 GP-FPLC Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.13 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.13
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.4.1.13 kidney
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.13 0.47
-
 in absence of 2-oxo-4-methylthiobutyric acid Rattus norvegicus
4.4.1.13 6.4
-
 in presence of 2-oxo-4-methylthiobutyric acid Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.13 S-(1,2-dichloroethenyl)-L-cysteine + H2O
-
 Rattus norvegicus pyruvate + NH3 + dichlorovinyl mercaptane
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.13 dimer 2 * 47400, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
4.4.1.13 More rat kidney enzyme: identical with rat kidney cytosolic glutamine transaminase K Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.4.1.13 37
-
 assay at Rattus norvegicus