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Literature summary extracted from

  • Tomisawa, H.; Suzuki, S.; Ichihara, S.; Fukazawa, H.; Tateishi, M.
    Purification and characterization of C-S lyase from Fusobacterium varium. A C-S cleavage enzyme of cysteine conjugates and some S-containing amino acids (1984), J. Biol. Chem., 259, 2588-2593.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.4.1.13 pyridoxal 5'-phosphate stabilizes Fusobacterium varium

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.13 hydroxylamine
-
 Fusobacterium varium
4.4.1.13 KCN
-
 Fusobacterium varium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.13 70000
-
 gel filtration, SDS-PAGE Fusobacterium varium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.13 additional information Fusobacterium varium intestinal microflora: formation of methylthio-containing metabolites ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.13 Fusobacterium varium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.13
-
 Fusobacterium varium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.13 additional information
-
-
 Fusobacterium varium

Storage Stability

EC Number Storage Stability Organism
4.4.1.13 -20°C, 7 months, no loss of activity Fusobacterium varium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.13 (S)-4-bromophenyl-L-cysteine
-
 Fusobacterium varium p-bromobenzenethiol + NH3 + pyruvate
-
 ?
4.4.1.13 additional information intestinal microflora: formation of methylthio-containing metabolites Fusobacterium varium ?
-
 ?
4.4.1.13 S-alkyl cysteines
-
 Fusobacterium varium ?
-
 ?
4.4.1.13 S-Aralkyl cysteines
-
 Fusobacterium varium ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.4.1.13 37
-
 assay at Fusobacterium varium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.13 7.5 8
-
 Fusobacterium varium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.4.1.13 5.5 9.5
-
 Fusobacterium varium