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Literature summary extracted from

  • Stevens, J.L.
    Isolation and characterization of a rat liver enzyme with both cysteine conjugate beta-lyase and kynureninase activity (1985), J. Biol. Chem., 260, 7945-7950.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.7.1.3 medicine NAD+ biosynthesis Rattus norvegicus

General Stability

EC Number General Stability Organism
4.4.1.13 pyridoxal 5'-phosphate stabilizes Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.13 3-hydroxykynurenine cysteine conjugate beta-lyase activity Rattus norvegicus
4.4.1.13 hydroxylamine
-
Rattus norvegicus
4.4.1.13 KCN
-
Rattus norvegicus
4.4.1.13 kynurenine cysteine conjugate beta-lyase activity Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7.1.3 0.013
-
L-3-hydroxykynurenine
-
Rattus norvegicus
3.7.1.3 0.4
-
L-kynurenine
-
Rattus norvegicus
4.4.1.13 0.013
-
3-hydroxy-kynurenine
-
Rattus norvegicus
4.4.1.13 0.4
-
kynurenine
-
Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.7.1.3 55000
-
2 * 55000, SDS-PAGE Rattus norvegicus
4.4.1.13 55000
-
2 * 55000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.7.1.3 3-hydroxy-L-kynurenine + H2O Rattus norvegicus
-
3-hydroxyanthranilate + L-Ala
-
?
3.7.1.3 L-kynurenine + H2O Rattus norvegicus
-
anthranilate + L-alanine
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.3 Rattus norvegicus
-
-
-
4.4.1.13 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.3
-
Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.7.1.3 liver
-
Rattus norvegicus
-
4.4.1.13 liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.7.1.3 0.25
-
-
Rattus norvegicus
4.4.1.13 0.0817
-
-
Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.3 3-hydroxy-L-kynurenine + H2O
-
Rattus norvegicus 3-hydroxyanthranilate + L-alanine
-
?
3.7.1.3 3-hydroxy-L-kynurenine + H2O
-
Rattus norvegicus 3-hydroxyanthranilate + L-Ala
-
?
3.7.1.3 L-kynurenine + H2O
-
Rattus norvegicus anthranilate + L-alanine
-
?
4.4.1.13 3-hydroxykynurenine
-
Rattus norvegicus ?
-
?
4.4.1.13 kynurenine
-
Rattus norvegicus ?
-
?
4.4.1.13 xenobiotics
-
Rattus norvegicus thiol + pyruvate + NH3
-
?

Subunits

EC Number Subunits Comment Organism
3.7.1.3 dimer 2 * 55000, SDS-PAGE Rattus norvegicus
4.4.1.13 dimer 2 * 55000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
4.4.1.13 More rat liver enzyme: same enzyme as kynureninase Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.4.1.13 70
-
50% loss of activity after 10 min Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
3.7.1.3 pyridoxal 5'-phosphate
-
Rattus norvegicus
4.4.1.13 pyridoxal 5'-phosphate
-
Rattus norvegicus