BRENDA - Enzyme Database

Coenzyme B12-dependent mutases causing carbon chain rearrangements

Barker, H.A.; The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ) 6, 509-537 (1972)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.99.4
1-Methyl-1,2-cis-cyclopropanedicarboxylate
noncompetitive, slight inhibitor
Eubacterium barkeri
5.4.99.4
1-Methyl-1,2-trans-cyclopropanedicarboxylate
competitive
Eubacterium barkeri
5.4.99.4
glutaconate
noncompetitive
Eubacterium barkeri
5.4.99.4
Itaconate
competitive
Eubacterium barkeri
5.4.99.4
L-malate
competitive
Eubacterium barkeri
5.4.99.4
mesaconate
competitive
Eubacterium barkeri
5.4.99.4
succinate
competitive
Eubacterium barkeri
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.99.1
0.5
-
3-methylaspartate
-
Clostridium cochlearium
5.4.99.1
0.5
-
3-methylaspartate
-
Clostridium tetanomorphum
5.4.99.1
1.5
-
L-Glu
-
Clostridium cochlearium
5.4.99.1
1.5
-
L-Glu
-
Clostridium tetanomorphum
5.4.99.4
7.1
-
2-Methyleneglutarate
-
Eubacterium barkeri
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.99.4
170000
-
sucrose density gradient centrifugation
Eubacterium barkeri
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.99.1
Glu
Rhodobacter sphaeroides
-
?
-
-
-
5.4.99.1
Glu
Rhodospirillum rubrum
-
?
-
-
-
5.4.99.1
Glu
Clostridium cochlearium
-
?
-
-
-
5.4.99.1
Glu
Clostridium tetanomorphum
-
?
-
-
-
5.4.99.4
additional information
Eubacterium barkeri
inducible enzyme, participates in the anaerobic conversion of nicotinic acid to equimolar amounts of propionate
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.99.1
Clostridium cochlearium
-
-
-
5.4.99.1
Clostridium tetanomorphum
-
-
-
5.4.99.1
Rhodobacter sphaeroides
-
-
-
5.4.99.1
Rhodospirillum rubrum
-
-
-
5.4.99.4
Eubacterium barkeri
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.4.99.1
purification of component E and component S
Clostridium tetanomorphum
5.4.99.4
-
Eubacterium barkeri
Reaction
EC Number
Reaction
Commentary
Organism
5.4.99.1
L-threo-3-methylaspartate = L-glutamate
mechanism
Clostridium tetanomorphum
5.4.99.4
2-Methyleneglutarate = 2-methylene-3-methylsuccinate
mechanism
Eubacterium barkeri
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
5.4.99.4
0.51
-
preparation which is heavily contaminated with methylitaconate isomerase
Eubacterium barkeri
Storage Stability
EC Number
Storage Stability
Organism
5.4.99.4
-80C, protein concentration 10 mg/ml, about 10% loss of activity after 6 months
Eubacterium barkeri
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.99.1
Glu
-
3453
Rhodobacter sphaeroides
?
-
-
-
-
5.4.99.1
Glu
-
3453
Rhodospirillum rubrum
?
-
-
-
-
5.4.99.1
Glu
-
3453
Clostridium cochlearium
?
-
-
-
-
5.4.99.1
Glu
-
3453
Clostridium tetanomorphum
?
-
-
-
-
5.4.99.1
L-Glu
-
3453
Clostridium cochlearium
threo-3-Methylaspartate
-
3453
Clostridium cochlearium
-
5.4.99.1
L-Glu
r
3453
Rhodobacter sphaeroides
threo-3-Methylaspartate
-
3453
Rhodobacter sphaeroides
-
5.4.99.1
L-Glu
r
3453
Rhodospirillum rubrum
threo-3-Methylaspartate
-
3453
Rhodospirillum rubrum
-
5.4.99.1
L-Glu
r
3453
Clostridium tetanomorphum
threo-3-Methylaspartate
-
3453
Clostridium tetanomorphum
-
5.4.99.1
L-Glu
equilibrium favours glutamate formation
3453
Rhodobacter sphaeroides
threo-3-Methylaspartate
-
3453
Rhodobacter sphaeroides
-
5.4.99.1
L-Glu
equilibrium favours glutamate formation
3453
Rhodospirillum rubrum
threo-3-Methylaspartate
-
3453
Rhodospirillum rubrum
-
5.4.99.1
L-Glu
equilibrium favours glutamate formation
3453
Clostridium tetanomorphum
threo-3-Methylaspartate
-
3453
Clostridium tetanomorphum
-
5.4.99.4
2-Methyleneglutarate
r
3453
Eubacterium barkeri
2-Methylene-3-methylsuccinate
i.e methylitaconate
3453
Eubacterium barkeri
-
5.4.99.4
additional information
inducible enzyme, participates in the anaerobic conversion of nicotinic acid to equimolar amounts of propionate
3453
Eubacterium barkeri
?
-
-
-
-
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
5.4.99.1
40
-
maximal activity in a 30 min incubation period
Clostridium tetanomorphum
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
5.4.99.1
55
-
rapidly inactivated
Clostridium tetanomorphum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4.99.1
8.5
-
-
Clostridium tetanomorphum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.4.99.1
Cobalamin
-
Clostridium cochlearium
5.4.99.1
Cobalamin
and at least seven analogs containing bases other than dimethylbenzimidazolyl can serve as coenzymes
Clostridium tetanomorphum
5.4.99.1
Cobalamin
-
Rhodobacter sphaeroides
5.4.99.1
Cobalamin
-
Rhodospirillum rubrum
5.4.99.4
cobamide
required
Eubacterium barkeri
5.4.99.4
vitamin B12
dependent on
Eubacterium barkeri
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.4.99.1
Cobalamin
-
Clostridium cochlearium
5.4.99.1
Cobalamin
and at least seven analogs containing bases other than dimethylbenzimidazolyl can serve as coenzymes
Clostridium tetanomorphum
5.4.99.1
Cobalamin
-
Rhodobacter sphaeroides
5.4.99.1
Cobalamin
-
Rhodospirillum rubrum
5.4.99.4
cobamide
required
Eubacterium barkeri
5.4.99.4
vitamin B12
dependent on
Eubacterium barkeri
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.99.4
1-Methyl-1,2-cis-cyclopropanedicarboxylate
noncompetitive, slight inhibitor
Eubacterium barkeri
5.4.99.4
1-Methyl-1,2-trans-cyclopropanedicarboxylate
competitive
Eubacterium barkeri
5.4.99.4
glutaconate
noncompetitive
Eubacterium barkeri
5.4.99.4
Itaconate
competitive
Eubacterium barkeri
5.4.99.4
L-malate
competitive
Eubacterium barkeri
5.4.99.4
mesaconate
competitive
Eubacterium barkeri
5.4.99.4
succinate
competitive
Eubacterium barkeri
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.99.1
0.5
-
3-methylaspartate
-
Clostridium cochlearium
5.4.99.1
0.5
-
3-methylaspartate
-
Clostridium tetanomorphum
5.4.99.1
1.5
-
L-Glu
-
Clostridium cochlearium
5.4.99.1
1.5
-
L-Glu
-
Clostridium tetanomorphum
5.4.99.4
7.1
-
2-Methyleneglutarate
-
Eubacterium barkeri
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.99.4
170000
-
sucrose density gradient centrifugation
Eubacterium barkeri
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.99.1
Glu
Rhodobacter sphaeroides
-
?
-
-
-
5.4.99.1
Glu
Rhodospirillum rubrum
-
?
-
-
-
5.4.99.1
Glu
Clostridium cochlearium
-
?
-
-
-
5.4.99.1
Glu
Clostridium tetanomorphum
-
?
-
-
-
5.4.99.4
additional information
Eubacterium barkeri
inducible enzyme, participates in the anaerobic conversion of nicotinic acid to equimolar amounts of propionate
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.99.1
purification of component E and component S
Clostridium tetanomorphum
5.4.99.4
-
Eubacterium barkeri
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
5.4.99.4
0.51
-
preparation which is heavily contaminated with methylitaconate isomerase
Eubacterium barkeri
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
5.4.99.4
-80C, protein concentration 10 mg/ml, about 10% loss of activity after 6 months
Eubacterium barkeri
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.99.1
Glu
-
3453
Rhodobacter sphaeroides
?
-
-
-
-
5.4.99.1
Glu
-
3453
Rhodospirillum rubrum
?
-
-
-
-
5.4.99.1
Glu
-
3453
Clostridium cochlearium
?
-
-
-
-
5.4.99.1
Glu
-
3453
Clostridium tetanomorphum
?
-
-
-
-
5.4.99.1
L-Glu
-
3453
Clostridium cochlearium
threo-3-Methylaspartate
-
3453
Clostridium cochlearium
-
5.4.99.1
L-Glu
r
3453
Rhodobacter sphaeroides
threo-3-Methylaspartate
-
3453
Rhodobacter sphaeroides
-
5.4.99.1
L-Glu
r
3453
Rhodospirillum rubrum
threo-3-Methylaspartate
-
3453
Rhodospirillum rubrum
-
5.4.99.1
L-Glu
r
3453
Clostridium tetanomorphum
threo-3-Methylaspartate
-
3453
Clostridium tetanomorphum
-
5.4.99.1
L-Glu
equilibrium favours glutamate formation
3453
Rhodobacter sphaeroides
threo-3-Methylaspartate
-
3453
Rhodobacter sphaeroides
-
5.4.99.1
L-Glu
equilibrium favours glutamate formation
3453
Rhodospirillum rubrum
threo-3-Methylaspartate
-
3453
Rhodospirillum rubrum
-
5.4.99.1
L-Glu
equilibrium favours glutamate formation
3453
Clostridium tetanomorphum
threo-3-Methylaspartate
-
3453
Clostridium tetanomorphum
-
5.4.99.4
2-Methyleneglutarate
r
3453
Eubacterium barkeri
2-Methylene-3-methylsuccinate
i.e methylitaconate
3453
Eubacterium barkeri
-
5.4.99.4
additional information
inducible enzyme, participates in the anaerobic conversion of nicotinic acid to equimolar amounts of propionate
3453
Eubacterium barkeri
?
-
-
-
-
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
5.4.99.1
40
-
maximal activity in a 30 min incubation period
Clostridium tetanomorphum
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
5.4.99.1
55
-
rapidly inactivated
Clostridium tetanomorphum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4.99.1
8.5
-
-
Clostridium tetanomorphum