BRENDA - Enzyme Database

beta-Methylaspartate-glutamate mutase from Clostridium tetanomorphum

Barker, H.A.; Methods Enzymol. 113, 121-133 (1985)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
5.4.99.1
2-mercaptoethanol
component S requires treatment with 2-mercaptoethanol to show maximal activity. Component E does not require this treatment
Clostridium tetanomorphum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.99.1
iodoacetate
component S is inhibited, component E is not inhibited
Clostridium tetanomorphum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.99.1
0.5
-
3-methylaspartate
-
Clostridium tetanomorphum
5.4.99.1
1.5
-
L-Glu
-
Clostridium tetanomorphum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.99.1
Glu
Clostridium tetanomorphum
initial reaction in anaerobic degradation of Glu
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.99.1
Clostridium tetanomorphum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.4.99.1
purification of component E and component S
Clostridium tetanomorphum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.99.1
Glu
initial reaction in anaerobic degradation of Glu
3452
Clostridium tetanomorphum
?
-
-
-
-
5.4.99.1
L-Glu
-
3452
Clostridium tetanomorphum
threo-3-Methylaspartate
-
3452
Clostridium tetanomorphum
-
Subunits
EC Number
Subunits
Commentary
Organism
5.4.99.1
?
-
Clostridium tetanomorphum
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
5.4.99.1
38
-
-
Clostridium tetanomorphum
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
5.4.99.1
27
38
27C: about 50% of maximal activity, 38C: maximal activity
Clostridium tetanomorphum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4.99.1
8.5
-
-
Clostridium tetanomorphum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.4.99.1
Cobalamin
and at least seven analogs containing bases other than dimethylbenzimidazolyl can serve as coenzymes
Clostridium tetanomorphum
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
5.4.99.1
2-mercaptoethanol
component S requires treatment with 2-mercaptoethanol to show maximal activity. Component E does not require this treatment
Clostridium tetanomorphum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.4.99.1
Cobalamin
and at least seven analogs containing bases other than dimethylbenzimidazolyl can serve as coenzymes
Clostridium tetanomorphum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.99.1
iodoacetate
component S is inhibited, component E is not inhibited
Clostridium tetanomorphum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.99.1
0.5
-
3-methylaspartate
-
Clostridium tetanomorphum
5.4.99.1
1.5
-
L-Glu
-
Clostridium tetanomorphum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.99.1
Glu
Clostridium tetanomorphum
initial reaction in anaerobic degradation of Glu
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.99.1
purification of component E and component S
Clostridium tetanomorphum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.99.1
Glu
initial reaction in anaerobic degradation of Glu
3452
Clostridium tetanomorphum
?
-
-
-
-
5.4.99.1
L-Glu
-
3452
Clostridium tetanomorphum
threo-3-Methylaspartate
-
3452
Clostridium tetanomorphum
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.4.99.1
?
-
Clostridium tetanomorphum
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
5.4.99.1
38
-
-
Clostridium tetanomorphum
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
5.4.99.1
27
38
27C: about 50% of maximal activity, 38C: maximal activity
Clostridium tetanomorphum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4.99.1
8.5
-
-
Clostridium tetanomorphum