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Literature summary extracted from

  • Chu, L.; Ebersole, J.L.; Kurzban, G.P.; Holt, S.C.
    Cystalysin, a 46-kDa L-cysteine desulfhydrase from Treponema denticola: biochemical and biophysical characterization (1999), Clin. Infect. Dis., 28, 442-450.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.4.1.1 2-mercaptoethanol increases activity Treponema denticola
4.4.1.28 2-mercaptoethanol 0.5 mM, enhances activity Treponema denticola

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.1 expression in Escherichia coli Treponema denticola
4.4.1.28 overexpressed in Escherichia coli LC-67 Treponema denticola

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.1 Ala competitive Treponema denticola
4.4.1.1 Gly competitive Treponema denticola
4.4.1.1 L-Asn weak Treponema denticola
4.4.1.1 L-Met weak Treponema denticola
4.4.1.1 L-Thr weak Treponema denticola
4.4.1.1 L-Val weak Treponema denticola
4.4.1.1 Ser competitive Treponema denticola
4.4.1.28 dithiothreitol 0.5 mM Treponema denticola
4.4.1.28 glycine
-
 Treponema denticola
4.4.1.28 L-alanine
-
 Treponema denticola
4.4.1.28 L-asparagine less effective inhibitor, Ki-value above 150 mM Treponema denticola
4.4.1.28 L-methionine less effective inhibitor, Ki-value above 150 mM Treponema denticola
4.4.1.28 L-serine
-
 Treponema denticola
4.4.1.28 L-threonine less effective inhibitor, Ki-value above 150 mM Treponema denticola
4.4.1.28 L-valine less effective inhibitor, Ki-value above 150 mM Treponema denticola
4.4.1.28 additional information not inhibited by Nalpha-p-tosyl-L-lysine chloromethyl ketone, pronase, proteinase K, CaCl2, MgCl2, ZnCl2, PMSF or benzamidine Treponema denticola

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.1 3.4
-
 Cys
-
 Treponema denticola
4.4.1.28 3.4
-
 L-cysteine pH 7.4, 22°C Treponema denticola

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.28 46000
-
 x * 46000, SDS-PAGE Treponema denticola

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.28 L-cysteine + H2O Treponema denticola
-
 sulfide + NH3 + pyruvate
-
 ?
4.4.1.28 L-cysteine + H2O Treponema denticola LC-67
-
 sulfide + NH3 + pyruvate
-
 ?
4.4.1.28 L-cysteine + H2O Treponema denticola ATCC 35404
-
 sulfide + NH3 + pyruvate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.1 Treponema denticola
-
 LC-67
-
4.4.1.1 Treponema denticola LC-67
-
 LC-67
-
4.4.1.28 Treponema denticola
-
-
-
4.4.1.28 Treponema denticola ATCC 35404
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.1
-
 Treponema denticola
4.4.1.28
-
 Treponema denticola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.1 L-Cys
-
 Treponema denticola H2S + NH3 + pyruvate
-
 ?
4.4.1.1 L-Cys
-
 Treponema denticola LC-67 H2S + NH3 + pyruvate
-
 ?
4.4.1.1 L-Cys
-
 Treponema denticola ATCC 35404 H2S + NH3 + pyruvate
-
 ?
4.4.1.1 L-cystathionine + H2O
-
 Treponema denticola L-homocysteine + pyruvate + NH3
-
 ?
4.4.1.1 L-cystathionine + H2O
-
 Treponema denticola LC-67 L-homocysteine + pyruvate + NH3
-
 ?
4.4.1.1 L-cystathionine + H2O
-
 Treponema denticola ATCC 35404 L-homocysteine + pyruvate + NH3
-
 ?
4.4.1.1 S-aminoethyl-L-Cys
-
 Treponema denticola ?
-
 ?
4.4.1.1 S-aminoethyl-L-Cys
-
 Treponema denticola LC-67 ?
-
 ?
4.4.1.1 S-aminoethyl-L-Cys
-
 Treponema denticola ATCC 35404 ?
-
 ?
4.4.1.28 cystathionine + H2O
-
 Treponema denticola NH3 + pyruvate + homocysteine
-
 ?
4.4.1.28 cystathionine + H2O
-
 Treponema denticola LC-67 NH3 + pyruvate + homocysteine
-
 ?
4.4.1.28 cystathionine + H2O
-
 Treponema denticola ATCC 35404 NH3 + pyruvate + homocysteine
-
 ?
4.4.1.28 L-cysteine + H2O
-
 Treponema denticola sulfide + NH3 + pyruvate
-
 ?
4.4.1.28 L-cysteine + H2O
-
 Treponema denticola LC-67 sulfide + NH3 + pyruvate
-
 ?
4.4.1.28 L-cysteine + H2O
-
 Treponema denticola ATCC 35404 sulfide + NH3 + pyruvate
-
 ?
4.4.1.28 S-aminoethyl-L-cysteine + H2O
-
 Treponema denticola NH3 + pyruvate + cysteamine
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.28 ? x * 46000, SDS-PAGE Treponema denticola

Synonyms

EC Number Synonyms Comment Organism
4.4.1.28 cystalysin
-
 Treponema denticola
4.4.1.28 L-cysteine desulfhydrase
-
 Treponema denticola

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.4.1.28 22
-
 assay at Treponema denticola

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.4.1.1 70
-
 30 min, complete inactivation Treponema denticola
4.4.1.28 50
-
 significant decrease in enzymatic activity above 50°C Treponema denticola
4.4.1.28 70
-
 30 min, complete inactivation Treponema denticola

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.1 12
-
 Cys
-
 Treponema denticola
4.4.1.28 12
-
 L-cysteine pH 7.4, 22°C Treponema denticola

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.1 7.8 8
-
 Treponema denticola
4.4.1.28 7.4
-
 assay at Treponema denticola

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.1 pyridoxal 5'-phosphate
-
 Treponema denticola

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.4.1.28 7.4
-
 glycine pH 7.4, 22°C Treponema denticola
4.4.1.28 15
-
 L-serine pH 7.4, 22°C Treponema denticola
4.4.1.28 22
-
 L-alanine pH 7.4, 22°C Treponema denticola

General Information

EC Number General Information Comment Organism
4.4.1.28 physiological function the enzyme could function in vivo as a virulence factor Treponema denticola