BRENDA - Enzyme Database

Glutamate mutase

Barker, H.A.; Methods Enzymol. 13, 319-330 (1969)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.99.1
0.5
-
3-methylaspartate
-
Clostridium tetanomorphum
5.4.99.1
1.5
-
L-Glu
-
Clostridium tetanomorphum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.99.1
Clostridium tetanomorphum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.4.99.1
purification of component E and component S
Clostridium tetanomorphum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.99.1
L-Glu
-
3450
Clostridium tetanomorphum
threo-3-Methylaspartate
-
3450
Clostridium tetanomorphum
-
Subunits
EC Number
Subunits
Commentary
Organism
5.4.99.1
?
the enzyme consists of two separable proteins called component E and component S, MW of component E determined by ultracentrifugation is 128000, the molecular weight of component S determined by ultracentrifugation is 17000
Clostridium tetanomorphum
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
5.4.99.1
35
40
-
Clostridium tetanomorphum
5.4.99.1
40
-
maximal activity in a 30 min incubation period
Clostridium tetanomorphum
5.4.99.1
55
-
maximal activity in an incubation period that is shorter than 30 min
Clostridium tetanomorphum
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
5.4.99.1
25
37
the reaction rate increases about 3fold when the temperature is increased from 25C to 37C
Clostridium tetanomorphum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4.99.1
8.5
-
-
Clostridium tetanomorphum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.4.99.1
Co-Deoxycobalamin
essential for the reaction
Clostridium tetanomorphum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.4.99.1
Co-Deoxycobalamin
essential for the reaction
Clostridium tetanomorphum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.99.1
0.5
-
3-methylaspartate
-
Clostridium tetanomorphum
5.4.99.1
1.5
-
L-Glu
-
Clostridium tetanomorphum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.99.1
purification of component E and component S
Clostridium tetanomorphum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.99.1
L-Glu
-
3450
Clostridium tetanomorphum
threo-3-Methylaspartate
-
3450
Clostridium tetanomorphum
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.4.99.1
?
the enzyme consists of two separable proteins called component E and component S, MW of component E determined by ultracentrifugation is 128000, the molecular weight of component S determined by ultracentrifugation is 17000
Clostridium tetanomorphum
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
5.4.99.1
35
40
-
Clostridium tetanomorphum
5.4.99.1
40
-
maximal activity in a 30 min incubation period
Clostridium tetanomorphum
5.4.99.1
55
-
maximal activity in an incubation period that is shorter than 30 min
Clostridium tetanomorphum
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
5.4.99.1
25
37
the reaction rate increases about 3fold when the temperature is increased from 25C to 37C
Clostridium tetanomorphum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4.99.1
8.5
-
-
Clostridium tetanomorphum