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Literature summary extracted from
- Glutamate mutase (1969), Methods Enzymol., 13, 319-330.
No PubMed abstract available
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.1 | 0.5 | - |
L-threo-3-methylaspartate | - |
Clostridium tetanomorphum |  |
| 5.4.99.1 | 1.5 | - |
L-Glu | - |
Clostridium tetanomorphum | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.1 | Clostridium tetanomorphum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.1 | purification of component E and component S | Clostridium tetanomorphum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.1 | L-Glu | - |
Clostridium tetanomorphum | threo-3-Methylaspartate | - |
? | |
| 5.4.99.1 | L-threo-3-methylaspartate | - |
Clostridium tetanomorphum | L-glutamate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.99.1 | ? | the enzyme consists of two separable proteins called component E and component S, MW of component E determined by ultracentrifugation is 128000, the molecular weight of component S determined by ultracentrifugation is 17000 | Clostridium tetanomorphum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.1 | 35 | 40 | - |
Clostridium tetanomorphum |
| 5.4.99.1 | 40 | - |
maximal activity in a 30 min incubation period | Clostridium tetanomorphum |
| 5.4.99.1 | 55 | - |
maximal activity in an incubation period that is shorter than 30 min | Clostridium tetanomorphum |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.1 | 25 | 37 | the reaction rate increases about 3fold when the temperature is increased from 25°C to 37°C | Clostridium tetanomorphum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.1 | 8.5 | - |
- |
Clostridium tetanomorphum |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.1 | Co-Deoxycobalamin | essential for the reaction | Clostridium tetanomorphum | |
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Release 2023.1 (January 2023)
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