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Literature summary extracted from

  • Pinto, R.M.; Faraldo, A.; Fernandez, A.; Canales, J.; Sillero, A.; Sillero, M.A.G.
    Adenylosuccinate lyase from Artemia embryos. Purification and properties (1983), J. Biol. Chem., 258, 12513-12519.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.2.2 AMP competitive Artemia sp.
4.3.2.2 fumarate noncompetitive Artemia sp.
4.3.2.2 KCl stimulates up to maximum concentration of 80 mM, inhibition at higher concentration Artemia sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.2.2 0.0012
-
 N6-(1,2-dicarboxyethyl)AMP
-
 Artemia sp.
4.3.2.2 0.035
-
 fumarate
-
 Artemia sp.
4.3.2.2 0.036
-
 AMP
-
 Artemia sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.2.2 KCl stimulates up to maximum concentration of 80 mM, inhibition at higher concentration Artemia sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.2.2 56000
-
 x * 56000, SDS-PAGE Artemia sp.
4.3.2.2 200000
-
 gel filtration Artemia sp.

Organism

EC Number Organism UniProt Comment Textmining
4.3.2.2 Artemia sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.2.2
-
 Artemia sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
4.3.2.2 N6-(1,2-dicarboxyethyl)AMP = fumarate + AMP uni bi-ordered mechanism Artemia sp.
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.3.2.2 cyst
-
 Artemia sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.2.2 2.4
-
-
 Artemia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.2.2 fumarate + AMP
-
 Artemia sp. N6-(1,2-dicarboxyethyl)AMP
-
 r
4.3.2.2 N6-(1,2-dicarboxyethyl)AMP i.e. adenylosuccinate Artemia sp. fumarate + AMP
-
 r

Subunits

EC Number Subunits Comment Organism
4.3.2.2 ? x * 56000, SDS-PAGE Artemia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.2.2 8 8.5
-
 Artemia sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.3.2.2 6 9.5 inactive below pH 6 and above 9.5 Artemia sp.