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Literature summary extracted from

  • Chakraborty, A.R.; Davidson, A.; Howell, P.L.
    Mutational analysis of amino acid residues involved in argininosuccinate lyase activity in duck delta II crystallin (1999), Biochemistry, 38, 2435-2443.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
4.3.2.1 E296Q retains only 0.001% residual activity Anas platyrhynchos
4.3.2.1 N116L no activity Anas platyrhynchos
4.3.2.1 R115N no activity Anas platyrhynchos
4.3.2.1 S283A no activity Anas platyrhynchos
4.3.2.1 S384A catalytic efficiency is decreased by an order of magnitude in comparison to wild-type delta II crystallin Anas platyrhynchos
4.3.2.1 T161V no activity Anas platyrhynchos
4.3.2.1 Y323F catalytic efficiency is decreased by an order of magnitude in comparison to wild-type delta II crystallin Anas platyrhynchos
4.3.2.1 Y323I no activity Anas platyrhynchos

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.2.1 0.02
-
 argininosuccinate mutant enzyme S284A Anas platyrhynchos
4.3.2.1 0.03
-
 argininosuccinate mutant enzyme R113N Anas platyrhynchos
4.3.2.1 0.07
-
 argininosuccinate wild-type enzyme Anas platyrhynchos
4.3.2.1 0.09
-
 argininosuccinate mutant enzyme S114A Anas platyrhynchos
4.3.2.1 0.4
-
 argininosuccinate
-
 Anas platyrhynchos
4.3.2.1 1.7
-
 argininosuccinate mutant enzyme Y323F Anas platyrhynchos

Organism

EC Number Organism UniProt Comment Textmining
4.3.2.1 Anas platyrhynchos
-
 wild type and mutant enzymes: S114A, R113N, S284A, Y323F, E296Q, r115N, N116L, T161V, S283A, and Y323I
-
4.3.2.1 Homo sapiens
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.3.2.1 2-(Nomega-L-arginino)succinate = fumarate + L-arginine 5 residues are essential for catalytic activity: R115, N116, T161, S283, and E296 Anas platyrhynchos
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.3.2.1 lens
-
 Anas platyrhynchos
-

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.2.1 0.12
-
 argininosuccinate mutant enzyme S284A Anas platyrhynchos
4.3.2.1 0.43
-
 argininosuccinate mutant enzyme Y323F Anas platyrhynchos
4.3.2.1 0.62
-
 argininosuccinate mutant enzyme R113N Anas platyrhynchos
4.3.2.1 2.42
-
 argininosuccinate mutant enzyme S114A Anas platyrhynchos
4.3.2.1 3.28
-
 argininosuccinate
-
 Homo sapiens
4.3.2.1 4.9
-
 argininosuccinate wild-type enzyme Anas platyrhynchos