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Literature summary extracted from

  • Kim, S.H.; Kronstad, J.W.; Ellis, B.E.
    Purification and characterization of phenylalanine ammonia-lyase from Ustilago maydis (1996), Phytochemistry, 43, 351-357.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.24 2-aminoindane-2-phosphonate
-
 Ustilago maydis
4.3.1.24 2-mercaptoethanol above 25 mM Ustilago maydis
4.3.1.24 8-hydroxyquinoline
-
 Ustilago maydis
4.3.1.24 Ag+
-
 Ustilago maydis
4.3.1.24 Cinnamic acid trans-cinnamate, moderately; trans-cinnamic acid Ustilago maydis
4.3.1.24 CN- NaCN Ustilago maydis
4.3.1.24 Cu2+
-
 Ustilago maydis
4.3.1.24 Hg2+
-
 Ustilago maydis
4.3.1.24 NaBH4
-
 Ustilago maydis
4.3.1.24 p-chloromercuribenzoate
-
 Ustilago maydis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.24 1.05
-
 L-Phe
-
 Ustilago maydis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.24 additional information no requirement for metal ion cofactors Ustilago maydis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.24 80000
-
 4 * 80000, SDS-PAGE Ustilago maydis
4.3.1.24 320000
-
 non-denaturing PAGE Ustilago maydis

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.24 Ustilago maydis
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.3.1.24 no modification no glycosylation Ustilago maydis

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.24
-
 Ustilago maydis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.24 additional information
-
-
 Ustilago maydis

Storage Stability

EC Number Storage Stability Organism
4.3.1.24 4°C, 5% loss of activity after 1 week, 40% loss of activity after 1 month Ustilago maydis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.24 L-Phe
-
 Ustilago maydis (E)-cinnamate + NH3
-
 ?
4.3.1.24 L-tyrosine no deamination of L-Tyr Ustilago maydis p-coumarate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.1.24 tetramer 4 * 80000, SDS-PAGE Ustilago maydis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.1.24 30
-
-
 Ustilago maydis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.3.1.24 50
-
 pH 7.0, 10 min, stable Ustilago maydis
4.3.1.24 60
-
 pH 7.0, 10 min, 30% loss of activity Ustilago maydis
4.3.1.24 70
-
 pH 7.0, 10 min, complete loss of activity Ustilago maydis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.24 8.8 9.2
-
 Ustilago maydis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.3.1.24 6.8 9.2 pH 6.5: 50% of maximal activity, pH 8.8-9.2: optimum Ustilago maydis