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Literature summary extracted from

  • Smith, M.A.; Grimm, B.
    Gabaculine resistance of Synechococcus glutamate 1-semialdehyde aminotransferase (1992), Biochemistry, 31, 4122-4127.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.3.8 wild-type enzyme and gabaculine-resistant mutant enzyme, overexpression in Escherichia coli Synechococcus sp.

Protein Variants

EC Number Protein Variants Comment Organism
5.4.3.8 additional information gabaculine-resistant mutant enzyme with a 3 times lower catalytic efficiency and impaired prototropic rearrangement and transaldimination Synechococcus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.3.8 gabaculine wild-type enzyme is inhibited, gabaculine-resistant mutant enzyme is not inhibited Synechococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.3.8 0.022
-
 (S)-4-Amino-5-oxopentanoate gabaculine-resistant mutant enzyme Synechococcus sp.
5.4.3.8 0.046
-
 (S)-4-Amino-5-oxopentanoate wild-type enzyme Synechococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
5.4.3.8 Synechococcus sp.
-
 wild-type strain and gabaculine-resistant strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.3.8 wild-type enzyme and gabaculine-resistant mutant enzyme Synechococcus sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
5.4.3.8 L-glutamate 1-semialdehyde = 5-aminolevulinate wild-type enzyme and gabaculine-resistant mutant enzyme use a ping-pong bi-bi mechanism in which 4,5-diaminovalerate is a likely intermediate Synechococcus sp.
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.3.8 (S)-4-Amino-5-oxopentanoate
-
 Synechococcus sp. 5-Amino-4-oxopentanoate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.3.8 0.077
-
 (S)-4-Amino-5-oxopentanoate gabaculine-resistant mutant enzyme Synechococcus sp.
5.4.3.8 0.47
-
 (S)-4-Amino-5-oxopentanoate wild-type enzyme Synechococcus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
5.4.3.8 vitamin B6 wild-type enzyme and gabaculine-resistant mutant enzyme contain vitamin B6 Synechococcus sp.