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Literature summary extracted from

  • Schindler, U.; Sans, N.; Schroeder, J.
    Ornithine cyclodeaminase from octopine Ti plasmid Ach5: identification, DNA sequence, enzyme properties, and comparison with gene and enzyme from nopaline Ti plasmid C58 (1989), J. Bacteriol., 171, 847-854.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.1.12 expression in Escherichia coli Agrobacterium tumefaciens
4.3.1.12 OCD, Arch5, and OCD, C58 Agrobacterium tumefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.12 additional information
-
 additional information
-
 Agrobacterium tumefaciens
4.3.1.12 0.25
-
 L-ornithine OCD, C58, in the presence of L-arginine Agrobacterium tumefaciens
4.3.1.12 0.25
-
 L-ornithine OCD, Ach5, in the absence of L-arginine Agrobacterium tumefaciens
4.3.1.12 1.7
-
 L-ornithine in the absence of L-arginine , OCD, C58 Agrobacterium tumefaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.12 39500
-
 2 * 39500, OCD, Ach5, calculation from sequence of amino acid Agrobacterium tumefaciens
4.3.1.12 42000
-
 2 * 42000, OCD, Ach5, SDS-PAGE Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.1.12 L-ornithine Agrobacterium tumefaciens
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.12 Agrobacterium tumefaciens
-
 C58, pTi nopaline
-
4.3.1.12 Agrobacterium tumefaciens
-
 Ach5, pTi octopine
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.12 additional information
-
-
 Agrobacterium tumefaciens

Storage Stability

EC Number Storage Stability Organism
4.3.1.12 -20°C, OCD, Arch5, and OCD, C58, stable for several months without loss of activity Agrobacterium tumefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.12 L-ornithine
-
 Agrobacterium tumefaciens L-proline + NH3
-
 ?
4.3.1.12 L-ornithine
-
 Agrobacterium tumefaciens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.1.12 dimer 2 * 39500, OCD, Ach5, calculation from sequence of amino acid Agrobacterium tumefaciens
4.3.1.12 dimer 2 * 42000, OCD, Ach5, SDS-PAGE Agrobacterium tumefaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.1.12 25 35 OCD, C58 Agrobacterium tumefaciens
4.3.1.12 25 35 in the absence of L-arginine Agrobacterium tumefaciens
4.3.1.12 30 35 OCD, Ach5 Agrobacterium tumefaciens
4.3.1.12 38 42 OCD, C58 Agrobacterium tumefaciens
4.3.1.12 38 42 in the presence of L-arginine Agrobacterium tumefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.12 8 8.5 OCD, Ach5 Agrobacterium tumefaciens
4.3.1.12 8 9.5 in the absence of L-arginine Agrobacterium tumefaciens
4.3.1.12 8 9.5 OCD, C58 Agrobacterium tumefaciens

Cofactor

EC Number Cofactor Comment Organism Structure
4.3.1.12 NAD+ required for maximal activity Agrobacterium tumefaciens