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Literature summary extracted from
- Ornithine cyclase (deaminating). II. Properties of the homogeneous enzyme (1974), J. Biol. Chem., 249, 7457-7462.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 4.3.1.12 | ornithine, NAD+ and dithiothreitol stabilize | Clostridium sporogenes |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.3.1.12 | iodoacetate | - |
Clostridium sporogenes |  |
| 4.3.1.12 | N-ethylmaleimide | 2 mM, complete inhibition | Clostridium sporogenes | |
| 4.3.1.12 | O2 | - |
Clostridium sporogenes | |
| 4.3.1.12 | p-chloromercuribenzoate | 1 mM, complete inhibition | Clostridium sporogenes | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.3.1.12 | 5.5 | - |
L-ornithine | in the absence of ADP | Clostridium sporogenes | |
| 4.3.1.12 | 6.2 | - |
L-ornithine | in the presence of ADP | Clostridium sporogenes | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.12 | 41500 | - |
2 * 41500, SDS-PAGE | Clostridium sporogenes |
| 4.3.1.12 | 80220 | - |
amino acid analysis | Clostridium sporogenes |
| 4.3.1.12 | 81000 | - |
equilibrium sedimentation | Clostridium sporogenes |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.3.1.12 | L-ornithine | Clostridium sporogenes | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.3.1.12 | Clostridium sporogenes | - |
National Canners Association, PA 3679 | - |
| 4.3.1.12 | Clostridium sporogenes | - |
ATCC 7955 | - |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 4.3.1.12 | addition of dithiothreitol and subsequent incubation in argon atmosphere completely reverses the inhibition caused by oxygen | Clostridium sporogenes |
| 4.3.1.12 | enzyme is sensitive to either pure oxygen or air | Clostridium sporogenes |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.3.1.12 | - |
Clostridium sporogenes |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.12 | additional information | - |
- |
Clostridium sporogenes |
| 4.3.1.12 | 6.84 | - |
- |
Clostridium sporogenes |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.3.1.12 | 4°C, 50% loss of activity under argon atmosphere within 3 days | Clostridium sporogenes |
| 4.3.1.12 | stable for at least 1 year when stored in sealed ampoules in argon atmosphere in liquid nitrogen | Clostridium sporogenes |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.3.1.12 | L-ornithine | anaerobic | Clostridium sporogenes | L-proline + NH3 | - |
? | |
| 4.3.1.12 | L-ornithine | - |
Clostridium sporogenes | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.3.1.12 | dimer | 2 * 41500, SDS-PAGE | Clostridium sporogenes |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.3.1.12 | ADP | stimulates | Clostridium sporogenes | |
| 4.3.1.12 | ATP | stimulates | Clostridium sporogenes | |
| 4.3.1.12 | NAD+ | tightly bound | Clostridium sporogenes | |
| 4.3.1.12 | NAD+ | 1 mol per mol of enzyme | Clostridium sporogenes | |
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