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Literature summary extracted from

  • Ertesvag, H.; Erlien, F.; Skjak-Braek, G.; Rehm, B.H.A.; Valla, S.
    Biochemical properties and substrate specificities of a recombinantly produced Azotobacter vinelandii alginate lyase (1998), J. Bacteriol., 180, 3779-3784.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.3 expression in Escherichia coli Azotobacter vinelandii

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.3 Zn2+ 1 mM ZnCl2 reduces the activity to 7.6% of the control level without Zn2+ added, 0.1 mM Zn2+ reduces the activity to 61% Azotobacter vinelandii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.3 0.46
-
 alginate
-
 Azotobacter vinelandii
4.2.2.3 0.46
-
 alginate alginate rich in mannuronic acid Azotobacter vinelandii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.3 NaCl activity is optimal at 0.35 M Azotobacter vinelandii
4.2.2.3 Zn2+ 1 mM ZnCl2, 92% loss of activity Azotobacter vinelandii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.3 39000
-
 x * 39000, SDS-PAGE Azotobacter vinelandii

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Azotobacter vinelandii
-
-
-
4.2.2.3 Azotobacter vinelandii O52195 alginate lyase precursor; recombinant
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.2.3 proteolytic modification signal peptide is cleaved off, leaving a mature protein of 39000 Da Azotobacter vinelandii

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.3 partial Azotobacter vinelandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 alginate alginate rich in mannuronic acids, the enzyme cleaves beta-D-mannuronic acid-beta-D-mannuronic acid bonds and beta-D-mannuronic acid-alpha-L-guluronic acid bonds but not alpha-L-guluronic acid-beta-D-mannuronic acid bonds and alpha-L-guluronic acid-alpha-L-guluronic acid-bonds Azotobacter vinelandii oligouronides
-
 ?
4.2.2.3 alginate enzyme cleaves beta-D-mannuronic acid-beta-D-mannuronic acid and beta-D-mannuronic acid-alpha-L-guluronic acid bonds but not alpha-L-guluronic acid-alpha-L-guluronic acid or alpha-L-guluronic acid-beta-D-mannuronic acid bonds Azotobacter vinelandii unsaturated algino-oligosaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.3 ? x * 39000, SDS-PAGE Azotobacter vinelandii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 8.1 8.4
-
 Azotobacter vinelandii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.3 6.7 8.4 pH 6.7: about 50% of maximal activity, pH 8.1-8.4: optimum Azotobacter vinelandii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.2.3 Azotobacter vinelandii isoelectric focusing
-
 5.1