Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dahms, A.S.; Sibley, D.; Huisman, W.; Donald, A.
    D-Galactonate dehydratase (1982), Methods Enzymol., 90, 294-298.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.6 iodoacetamide
-
 Pseudomonas sp.
4.2.1.6 PCMB
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.6 1.14
-
 D-galactonate
-
 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.6 Mg2+ optimal concentration: 1 mM Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.6 240000 245000
-
 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.6 D-galactonate Pseudomonas sp. induced by growth on D-galactose, D-galactonate, D-fucose, and L-arabinose over the level present in glucose-grown cells ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.6 Pseudomonas sp.
-
 ATCC 27855
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.6
-
 Pseudomonas sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.6 D-galactonate = 2-dehydro-3-deoxy-D-galactonate + H2O the immediate dehydration product is the enol which undergoes spontaneous ketonization Pseudomonas sp.
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.6 38
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
4.2.1.6 -20°C, 0.05 M glycine-0.01 M sodium cacodylate, pH 8.8, stable for 6 months Pseudomonas sp.
4.2.1.6 -20°C, 6 months, stable Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.6 D-galactonate induced by growth on D-galactose, D-galactonate, D-fucose, and L-arabinose over the level present in glucose-grown cells Pseudomonas sp. ?
-
 ?
4.2.1.6 D-galactonate the enzyme is strictly specific for D-galactonate Pseudomonas sp. 2-dehydro-3-deoxy-D-galactonate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.6 tetramer
-
 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.6 7.1
-
-
 Pseudomonas sp.