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Literature summary extracted from
- Purification and properties of D-galactonate dehydratase from Mycobacterium butyricum (1981), Biochim. Biophys. Acta, 661, 240-246.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.6 | CdSO4 | - |
Mycobacterium butyricum |  |
| 4.2.1.6 | CuSO4 | - |
Mycobacterium butyricum | |
| 4.2.1.6 | HgCl2 | - |
Mycobacterium butyricum | |
| 4.2.1.6 | iodoacetate | - |
Mycobacterium butyricum | |
| 4.2.1.6 | NaF | - |
Mycobacterium butyricum | |
| 4.2.1.6 | PCMB | - |
Mycobacterium butyricum | |
| 4.2.1.6 | ZnSO4 | reactivated by chelating agents | Mycobacterium butyricum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.6 | 1 | - |
D-galactonate | - |
Mycobacterium butyricum | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.6 | Mg2+ | highest activity at 0.3 mM MgCl2, Mg2+ or Mn2+ required | Mycobacterium butyricum | |
| 4.2.1.6 | Mn2+ | highest activity at 0.02 mM MnCl2, Mn2+ or Mg2+ required | Mycobacterium butyricum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.6 | 270000 | - |
gel filtration | Mycobacterium butyricum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.6 | D-galactonate | Mycobacterium butyricum | the enzyme catalyzes the first reaction in the D-galactonate catabolic pathway of non-pathogenic mycobacteria | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.6 | Mycobacterium butyricum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.6 | - |
Mycobacterium butyricum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.6 | 4.56 | - |
- |
Mycobacterium butyricum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.6 | D-galactonate | the enzyme catalyzes the first reaction in the D-galactonate catabolic pathway of non-pathogenic mycobacteria | Mycobacterium butyricum | ? | - |
? | |
| 4.2.1.6 | D-galactonate | the enzyme is strictly specific for D-galactonate | Mycobacterium butyricum | 2-dehydro-3-deoxy-D-galactonate + H2O | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.6 | additional information | - |
dithiothreitol increases heat sensitivity | Mycobacterium butyricum |
| 4.2.1.6 | 70 | - |
15 min, about 10% loss of activity | Mycobacterium butyricum |
| 4.2.1.6 | 75 | - |
10 min, about 60% loss of activity. 15 min, about 90% loss of activity | Mycobacterium butyricum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.6 | 7.8 | 8 | in 100 mM tris-HCl buffer or 50 mM phosphate buffer | Mycobacterium butyricum |
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