BRENDA - Enzyme Database show

Structure of dihydrodipicolinate synthase of Nicotiana sylvestris reveals novel quaternary structure

Blickling, S.; Beisel, H.G.; Bozic, D.; Knaeblein, J.; Laber, B.; Huber, R.; J. Mol. Biol. 274, 608-621 (1997)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
4.3.3.7
crystal structure at 2.8 A resolution
Nicotiana sylvestris
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.3.7
L-lysine
discussion of plant inhibition versus bacterial inhibition
Nicotiana sylvestris
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.3.7
Nicotiana sylvestris
-
tobacco
-
Reaction
EC Number
Reaction
Commentary
Organism
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
the reaction is initiated by an nucleophilic attack of the epsilon-amino group of active site lysine to the C2 atom of pyruvate with subsequent imine formation. Tautomerization of the imine to the enamine creates the nucleophile necessary for the attack of the aldehyde group of L-aspartate semialdehyde. Cyclization of the substrate coupled with transamination leads to stepwise detachment from the active site
Nicotiana sylvestris
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
-
33921
Nicotiana sylvestris
dihydrodipicolinate + H2O
(4S)-4-hydroxy-2,3,4,5-terahydro-(2S)-dipicolinic acid is the initial but instable product
33921
Nicotiana sylvestris
?
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
4.3.3.7
crystal structure at 2.8 A resolution
Nicotiana sylvestris
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.3.7
L-lysine
discussion of plant inhibition versus bacterial inhibition
Nicotiana sylvestris
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
-
33921
Nicotiana sylvestris
dihydrodipicolinate + H2O
(4S)-4-hydroxy-2,3,4,5-terahydro-(2S)-dipicolinic acid is the initial but instable product
33921
Nicotiana sylvestris
?