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Literature summary extracted from

  • Dereppe, C.; Bold, G.; Ghisalba, O.; Ebert, E.; Schaer, H.P.
    Purification and characterization of dihydrodipicolinate synthase from pea (1991), Plant Physiol., 98, 813-821.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.3.3.7 (2R,3S,6S)-2,6-diamino-3-hydroxyheptanedioic acid 134% of the activity without Pisum sativum
4.3.3.7 (2R,6S)-2,6-diamino-2-methyl-3-heptenedioic acid 110% of the activity without Pisum sativum
4.3.3.7 (2R,6S/S)-2,6-diamino-6-phosphonohexanoic acid 113% of the activity without Pisum sativum

General Stability

EC Number General Stability Organism
4.3.3.7 unstable at all stages of purification, not stabilized by dithioerythritol, glycerol, KCl, (NH4)2SO4, sucrose, divalent cations Pisum sativum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.3.7 3-Bromopyruvate
-
 Pisum sativum
4.3.3.7 3-bromopyruvate ethyl ester 1.2 mM, 85% inhibition Pisum sativum
4.3.3.7 DL-diaminopimelic acid 1.2 mM, 91% inhibition Pisum sativum
4.3.3.7 L-alpha-(2-aminoethoxyvinyl)-glycine 1.2 mM, 100% inhibition Pisum sativum
4.3.3.7 L-arginine 1.2 mM, 62% inhibition Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.3.7 0.4
-
 L-aspartate-4-semialdehyde
-
 Pisum sativum
4.3.3.7 1.7
-
 pyruvate
-
 Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.3.7 41000
-
 3 * 41000, SDS-PAGE Pisum sativum
4.3.3.7 127000
-
 gel filtration Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.7 Pisum sativum
-
 pea
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.3.7
-
 Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.3.3.7 seedling
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.3.7 additional information
-
-
 Pisum sativum

Storage Stability

EC Number Storage Stability Organism
4.3.3.7 -20°C, 15% loss of activity after 4 d Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.7 L-aspartate-4-semialdehyde + pyruvate
-
 Pisum sativum dihydrodipicolinate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.3.7 trimer 3 * 41000, SDS-PAGE Pisum sativum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.3.7 35
-
-
 Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.3.7 8
-
-
 Pisum sativum