BRENDA - Enzyme Database show

Purification and characterization of dihydrodipicolinate synthase from pea

Dereppe, C.; Bold, G.; Ghisalba, O.; Ebert, E.; Schaer, H.P.; Plant Physiol. 98, 813-821 (1991)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.3.3.7
(2R,3S,6S)-2,6-diamino-3-hydroxyheptanedioic acid
134% of the activity without
Pisum sativum
4.3.3.7
(2R,6S)-2,6-diamino-2-methyl-3-heptenedioic acid
110% of the activity without
Pisum sativum
4.3.3.7
(2R,6S/S)-2,6-diamino-6-phosphonohexanoic acid
113% of the activity without
Pisum sativum
General Stability
EC Number
General Stability
Organism
4.3.3.7
unstable at all stages of purification, not stabilized by dithioerythritol, glycerol, KCl, (NH4)2SO4, sucrose, divalent cations
Pisum sativum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.3.7
3-Bromopyruvate
-
Pisum sativum
4.3.3.7
3-bromopyruvate ethyl ester
1.2 mM, 85% inhibition
Pisum sativum
4.3.3.7
DL-diaminopimelic acid
1.2 mM, 91% inhibition
Pisum sativum
4.3.3.7
L-alpha-(2-aminoethoxyvinyl)-glycine
1.2 mM, 100% inhibition
Pisum sativum
4.3.3.7
L-arginine
1.2 mM, 62% inhibition
Pisum sativum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.3.7
0.4
-
L-aspartate-4-semialdehyde
-
Pisum sativum
4.3.3.7
1.7
-
pyruvate
-
Pisum sativum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.3.7
41000
-
3 * 41000, SDS-PAGE
Pisum sativum
4.3.3.7
127000
-
gel filtration
Pisum sativum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.3.7
Pisum sativum
-
pea
-
Purification (Commentary)
EC Number
Commentary
Organism
4.3.3.7
-
Pisum sativum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.3.3.7
seedling
-
Pisum sativum
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3.3.7
additional information
-
-
Pisum sativum
Storage Stability
EC Number
Storage Stability
Organism
4.3.3.7
-20°C, 15% loss of activity after 4 d
Pisum sativum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
-
33915
Pisum sativum
dihydrodipicolinate + H2O
-
33915
Pisum sativum
?
Subunits
EC Number
Subunits
Commentary
Organism
4.3.3.7
trimer
3 * 41000, SDS-PAGE
Pisum sativum
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.3.3.7
35
-
-
Pisum sativum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3.3.7
8
-
-
Pisum sativum
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.3.3.7
(2R,3S,6S)-2,6-diamino-3-hydroxyheptanedioic acid
134% of the activity without
Pisum sativum
4.3.3.7
(2R,6S)-2,6-diamino-2-methyl-3-heptenedioic acid
110% of the activity without
Pisum sativum
4.3.3.7
(2R,6S/S)-2,6-diamino-6-phosphonohexanoic acid
113% of the activity without
Pisum sativum
General Stability (protein specific)
EC Number
General Stability
Organism
4.3.3.7
unstable at all stages of purification, not stabilized by dithioerythritol, glycerol, KCl, (NH4)2SO4, sucrose, divalent cations
Pisum sativum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.3.7
3-Bromopyruvate
-
Pisum sativum
4.3.3.7
3-bromopyruvate ethyl ester
1.2 mM, 85% inhibition
Pisum sativum
4.3.3.7
DL-diaminopimelic acid
1.2 mM, 91% inhibition
Pisum sativum
4.3.3.7
L-alpha-(2-aminoethoxyvinyl)-glycine
1.2 mM, 100% inhibition
Pisum sativum
4.3.3.7
L-arginine
1.2 mM, 62% inhibition
Pisum sativum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.3.7
0.4
-
L-aspartate-4-semialdehyde
-
Pisum sativum
4.3.3.7
1.7
-
pyruvate
-
Pisum sativum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.3.7
41000
-
3 * 41000, SDS-PAGE
Pisum sativum
4.3.3.7
127000
-
gel filtration
Pisum sativum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.3.7
-
Pisum sativum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.3.3.7
seedling
-
Pisum sativum
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3.3.7
additional information
-
-
Pisum sativum
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.3.3.7
-20°C, 15% loss of activity after 4 d
Pisum sativum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
-
33915
Pisum sativum
dihydrodipicolinate + H2O
-
33915
Pisum sativum
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.3.3.7
trimer
3 * 41000, SDS-PAGE
Pisum sativum
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.3.3.7
35
-
-
Pisum sativum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3.3.7
8
-
-
Pisum sativum