BRENDA - Enzyme Database show

Isolation and characterization of dihydrodipicolinate synthase from maize

Frisch, D.A.; Gengenbach, B.G.; Tommy, A.M.; Sellner, J.M.; Somers, D.A.; Myers, D.E.; Plant Physiol. 96, 444-452 (1991)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.3.7
L-lysine
-
Zea mays
4.3.3.7
lysine ethyl ester
-
Zea mays
4.3.3.7
PMSF
-
Zea mays
4.3.3.7
S-(2-aminoethyl)-L-cysteine
-
Zea mays
4.3.3.7
threo-4-hydroxy-L-lysine
-
Zea mays
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.3.7
0.6
-
L-aspartate-4-semialdehyde
-
Zea mays
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.3.7
72000
-
4 * 37000-38000 + x * 72000, the 72000 Da subunit possibly modifies the structure and kinetic properties, SDS-PAGE
Zea mays
4.3.3.7
130000
-
non-denaturing PAGE
Zea mays
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
Zea mays
-
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.3.7
Zea mays
-
corn
-
Purification (Commentary)
EC Number
Commentary
Organism
4.3.3.7
-
Zea mays
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.3.3.7
cell suspension culture
-
Zea mays
-
4.3.3.7
leaf
-
Zea mays
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.3.3.7
additional information
-
-
Zea mays
Storage Stability
EC Number
Storage Stability
Organism
4.3.3.7
4░C, 20 mM bis-Tris propane buffer, pH 8.0, 10 mM pyruvate, 300 mM NaCl, 0.01% NaN3, 3 weeks, 20% loss of activity
Zea mays
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
-
33911
Zea mays
dihydrodipicolinate + H2O
-
33911
Zea mays
?
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
-
33911
Zea mays
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.3.3.7
?
alpha,beta or alpha2beta
Zea mays
4.3.3.7
tetramer
4 * 37000-38000 + x * 72000, the 72000 Da subunit possibly modifies the structure and kinetic properties, SDS-PAGE
Zea mays
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.3.7
L-lysine
-
Zea mays
4.3.3.7
lysine ethyl ester
-
Zea mays
4.3.3.7
PMSF
-
Zea mays
4.3.3.7
S-(2-aminoethyl)-L-cysteine
-
Zea mays
4.3.3.7
threo-4-hydroxy-L-lysine
-
Zea mays
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.3.7
0.6
-
L-aspartate-4-semialdehyde
-
Zea mays
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.3.7
72000
-
4 * 37000-38000 + x * 72000, the 72000 Da subunit possibly modifies the structure and kinetic properties, SDS-PAGE
Zea mays
4.3.3.7
130000
-
non-denaturing PAGE
Zea mays
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
Zea mays
-
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.3.7
-
Zea mays
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.3.3.7
cell suspension culture
-
Zea mays
-
4.3.3.7
leaf
-
Zea mays
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.3.3.7
additional information
-
-
Zea mays
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.3.3.7
4░C, 20 mM bis-Tris propane buffer, pH 8.0, 10 mM pyruvate, 300 mM NaCl, 0.01% NaN3, 3 weeks, 20% loss of activity
Zea mays
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
-
33911
Zea mays
dihydrodipicolinate + H2O
-
33911
Zea mays
?
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
-
33911
Zea mays
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.3.3.7
?
alpha,beta or alpha2beta
Zea mays
4.3.3.7
tetramer
4 * 37000-38000 + x * 72000, the 72000 Da subunit possibly modifies the structure and kinetic properties, SDS-PAGE
Zea mays