BRENDA - Enzyme Database show

Partial purification and characterization of dihydrodipicolinic acid synthetase from sporulating Bacillus megaterium

Webster, F.H.; Lechowich, R.V.; J. Bacteriol. 101, 118-126 (1970)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.3.3.7
2-mercaptoethanol
activation
Bacillus megaterium
4.3.3.7
cysteine
activation
Bacillus megaterium
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.3.7
L-lysine
no inhibition
Bacillus megaterium
4.3.3.7
additional information
not inhibitory: iodoacetate, iodoacetamide, p-chloromercuribenzoate
Bacillus megaterium
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.3.7
0.46
-
L-aspartate-4-semialdehyde
-
Bacillus megaterium
4.3.3.7
0.5
-
pyruvate
-
Bacillus megaterium
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.3.7
Bacillus megaterium
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.3.3.7
partial
Bacillus megaterium
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.3.3.7
sporulating cell
-
Bacillus megaterium
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3.3.7
additional information
-
-
Bacillus megaterium
Storage Stability
EC Number
Storage Stability
Organism
4.3.3.7
-20°C, pH 6.5
Bacillus megaterium
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
-
33908
Bacillus megaterium
dihydrodipicolinate + H2O
-
33908
Bacillus megaterium
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.3.3.7
60
-
inactivation energy: 67300 cal/mol, change in free energy, DELTAF: 22500 cal/mol, change in enthalpy, DELTAH: 66700 cal/mol, change in entropy DELTAS: 133 cal/degree/mol
Bacillus megaterium
4.3.3.7
75
-
pyruvate increases the half-life from 2.7 to 18.3 min
Bacillus megaterium
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3.3.7
7.7
-
-
Bacillus megaterium
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
4.3.3.7
6.5
8
pH 6.5, half-life: 5.3 min, pH 8..0, half-life: 20 min
Bacillus megaterium
4.3.3.7
10.5
-
maximal thermal stability at this pH
Bacillus megaterium
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.3.3.7
2-mercaptoethanol
activation
Bacillus megaterium
4.3.3.7
cysteine
activation
Bacillus megaterium
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.3.7
L-lysine
no inhibition
Bacillus megaterium
4.3.3.7
additional information
not inhibitory: iodoacetate, iodoacetamide, p-chloromercuribenzoate
Bacillus megaterium
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.3.7
0.46
-
L-aspartate-4-semialdehyde
-
Bacillus megaterium
4.3.3.7
0.5
-
pyruvate
-
Bacillus megaterium
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.3.7
partial
Bacillus megaterium
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.3.3.7
sporulating cell
-
Bacillus megaterium
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3.3.7
additional information
-
-
Bacillus megaterium
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.3.3.7
-20°C, pH 6.5
Bacillus megaterium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
-
33908
Bacillus megaterium
dihydrodipicolinate + H2O
-
33908
Bacillus megaterium
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.3.3.7
60
-
inactivation energy: 67300 cal/mol, change in free energy, DELTAF: 22500 cal/mol, change in enthalpy, DELTAH: 66700 cal/mol, change in entropy DELTAS: 133 cal/degree/mol
Bacillus megaterium
4.3.3.7
75
-
pyruvate increases the half-life from 2.7 to 18.3 min
Bacillus megaterium
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3.3.7
7.7
-
-
Bacillus megaterium
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
4.3.3.7
6.5
8
pH 6.5, half-life: 5.3 min, pH 8..0, half-life: 20 min
Bacillus megaterium
4.3.3.7
10.5
-
maximal thermal stability at this pH
Bacillus megaterium