BRENDA - Enzyme Database show

Dihydrodipicolinic acid synthase of Bacillus licheniformis. Quaternary structure, kinetics, and stability in the presence of sodium chloride and substrates

Halling, S.M.; Stahly, D.P.; Biochim. Biophys. Acta 452, 580-596 (1976)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.3.7
2.5
3
pyruvate
-
Bacillus licheniformis
4.3.3.7
5
-
L-aspartate-4-semialdehyde
-
Bacillus licheniformis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.3.7
28000
-
4 * 28000, SDS-PAGE
Bacillus licheniformis
4.3.3.7
108000
117000
gel filtration
Bacillus licheniformis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.3.7
Bacillus licheniformis
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.3.3.7
-
Bacillus licheniformis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3.3.7
additional information
-
-
Bacillus licheniformis
Storage Stability
EC Number
Storage Stability
Organism
4.3.3.7
2-4°C
Bacillus licheniformis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
-
33905
Bacillus licheniformis
dihydrodipicolinate + H2O
-
33905
Bacillus licheniformis
?
Subunits
EC Number
Subunits
Commentary
Organism
4.3.3.7
tetramer
4 * 28000, SDS-PAGE
Bacillus licheniformis
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.3.3.7
74
79
inactivation energy: 117000 cal/mol, change in free energy, DELTAF: 23400 cal/mol, change in enthalpy, DELTAH: 113500 cal/mol, change in entropy DELTAS: 259 cal/degre e/mol
Bacillus licheniformis
4.3.3.7
80
-
no reactivation after heating to 80°C, pyruvate increases thermostability
Bacillus licheniformis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.3.7
2.5
3
pyruvate
-
Bacillus licheniformis
4.3.3.7
5
-
L-aspartate-4-semialdehyde
-
Bacillus licheniformis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.3.7
28000
-
4 * 28000, SDS-PAGE
Bacillus licheniformis
4.3.3.7
108000
117000
gel filtration
Bacillus licheniformis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.3.7
-
Bacillus licheniformis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3.3.7
additional information
-
-
Bacillus licheniformis
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.3.3.7
2-4°C
Bacillus licheniformis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
-
33905
Bacillus licheniformis
dihydrodipicolinate + H2O
-
33905
Bacillus licheniformis
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.3.3.7
tetramer
4 * 28000, SDS-PAGE
Bacillus licheniformis
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.3.3.7
74
79
inactivation energy: 117000 cal/mol, change in free energy, DELTAF: 23400 cal/mol, change in enthalpy, DELTAH: 113500 cal/mol, change in entropy DELTAS: 259 cal/degre e/mol
Bacillus licheniformis
4.3.3.7
80
-
no reactivation after heating to 80°C, pyruvate increases thermostability
Bacillus licheniformis