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Literature summary extracted from
- Dihydrodipicolinic acid synthase of Bacillus licheniformis. Quaternary structure, kinetics, and stability in the presence of sodium chloride and substrates (1976), Biochim. Biophys. Acta, 452, 580-596.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.3.3.7 | 2.5 | 3 | pyruvate | - |
Bacillus licheniformis |  |
| 4.3.3.7 | 5 | - |
L-aspartate-4-semialdehyde | - |
Bacillus licheniformis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.3.3.7 | 28000 | - |
4 * 28000, SDS-PAGE | Bacillus licheniformis |
| 4.3.3.7 | 108000 | 117000 | gel filtration | Bacillus licheniformis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.3.3.7 | Bacillus licheniformis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.3.3.7 | - |
Bacillus licheniformis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.3.3.7 | additional information | - |
- |
Bacillus licheniformis |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.3.3.7 | 2-4°C | Bacillus licheniformis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.3.3.7 | L-aspartate-4-semialdehyde + pyruvate | - |
Bacillus licheniformis | dihydrodipicolinate + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.3.3.7 | tetramer | 4 * 28000, SDS-PAGE | Bacillus licheniformis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.3.3.7 | 74 | 79 | inactivation energy: 117000 cal/mol, change in free energy, DELTAF: 23400 cal/mol, change in enthalpy, DELTAH: 113500 cal/mol, change in entropy DELTAS: 259 cal/degre e/mol | Bacillus licheniformis |
| 4.3.3.7 | 80 | - |
no reactivation after heating to 80°C, pyruvate increases thermostability | Bacillus licheniformis |
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