BRENDA - Enzyme Database show

Spinach leaf dihydrodipicolinate synthase: partial purification and characterization

Wallsgrove, R.M.; Mazelis, M.; Phytochemistry 20, 2651-2655 (1981)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.3.7
isoleucine
5 mM, significant inhibition
Spinacia oleracea
4.3.3.7
L-arginine
-
Pisum sativum
4.3.3.7
L-arginine
5 mM, significant inhibition
Spinacia oleracea
4.3.3.7
L-lysine
0.1 mM, complete inhibition, I0.5: 0.02 mM
Spinacia oleracea
4.3.3.7
S-(2-aminoethyl)-L-cysteine
-
Pisum sativum
4.3.3.7
S-(2-aminoethyl)-L-cysteine
I0.5: 0.4 mM
Spinacia oleracea
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.3.7
1
-
L-aspartate-4-semialdehyde
-
Spinacia oleracea
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.3.7
115000
-
gel filtration
Spinacia oleracea
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
Spinacia oleracea
-
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.3.7
Pisum sativum
-
pea
-
4.3.3.7
Spinacia oleracea
-
spinach
-
Purification (Commentary)
EC Number
Commentary
Organism
4.3.3.7
partial
Spinacia oleracea
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.3.3.7
leaf
-
Spinacia oleracea
-
Storage Stability
EC Number
Storage Stability
Organism
4.3.3.7
-20C, 25% loss of activity per week
Spinacia oleracea
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
-
33903
Spinacia oleracea
dihydrodipicolinate + H2O
-
33903
Spinacia oleracea
?
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
-
33903
Pisum sativum
dihydrodipicolinate + H2O
-
33903
Pisum sativum
?
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
-
33903
Spinacia oleracea
?
-
-
-
-
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.3.7
isoleucine
5 mM, significant inhibition
Spinacia oleracea
4.3.3.7
L-arginine
-
Pisum sativum
4.3.3.7
L-arginine
5 mM, significant inhibition
Spinacia oleracea
4.3.3.7
L-lysine
0.1 mM, complete inhibition, I0.5: 0.02 mM
Spinacia oleracea
4.3.3.7
S-(2-aminoethyl)-L-cysteine
-
Pisum sativum
4.3.3.7
S-(2-aminoethyl)-L-cysteine
I0.5: 0.4 mM
Spinacia oleracea
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.3.7
1
-
L-aspartate-4-semialdehyde
-
Spinacia oleracea
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.3.7
115000
-
gel filtration
Spinacia oleracea
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
Spinacia oleracea
-
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.3.7
partial
Spinacia oleracea
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.3.3.7
leaf
-
Spinacia oleracea
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.3.3.7
-20C, 25% loss of activity per week
Spinacia oleracea
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
-
33903
Spinacia oleracea
dihydrodipicolinate + H2O
-
33903
Spinacia oleracea
?
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
-
33903
Pisum sativum
dihydrodipicolinate + H2O
-
33903
Pisum sativum
?
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
-
33903
Spinacia oleracea
?
-
-
-
-