BRENDA - Enzyme Database show

Purification and characterization of dihydrodipicolinate synthase from wheat suspension cultures

Kumpaisal, R.; Hashimoto, T.; Yamada, Y.; Plant Physiol. 85, 145-151 (1987)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.3.7
DL-diaminopimelic acid
slightly inhibitory
Triticum aestivum
4.3.3.7
KCl
1 M, 35% inactivation, 2 M, 55% inactivation
Triticum aestivum
4.3.3.7
L-arginine
slightly inhibitory
Triticum aestivum
4.3.3.7
L-lysine
-
Triticum aestivum
4.3.3.7
S-(2-aminoethyl)-L-cysteine
-
Triticum aestivum
4.3.3.7
threo-4-hydroxy-L-lysine
-
Triticum aestivum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.3.7
0.8
-
L-aspartate-4-semialdehyde
-
Triticum aestivum
4.3.3.7
11.7
-
pyruvate
-
Triticum aestivum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.3.7
123000
-
gel filtration
Triticum aestivum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
Triticum aestivum
biosynthesis of lysine via the diaminopimelate pathway
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.3.7
Triticum aestivum
-
wheat
-
Purification (Commentary)
EC Number
Commentary
Organism
4.3.3.7
-
Triticum aestivum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.3.3.7
cell suspension culture
-
Triticum aestivum
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.3.3.7
additional information
-
-
Triticum aestivum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
D-isomer inactive
33902
Triticum aestivum
dihydrodipicolinate + H2O
-
33902
Triticum aestivum
?
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
biosynthesis of lysine via the diaminopimelate pathway
33902
Triticum aestivum
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.3.3.7
tetramer
4 * 32000-34000, SDS-PAGE
Triticum aestivum
Temperature Stability [░C]
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
4.3.3.7
60
-
1 min, 50% loss of activity, 5 min, complete inactivation
Triticum aestivum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3.3.7
8
-
-
Triticum aestivum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.3.7
DL-diaminopimelic acid
slightly inhibitory
Triticum aestivum
4.3.3.7
KCl
1 M, 35% inactivation, 2 M, 55% inactivation
Triticum aestivum
4.3.3.7
L-arginine
slightly inhibitory
Triticum aestivum
4.3.3.7
L-lysine
-
Triticum aestivum
4.3.3.7
S-(2-aminoethyl)-L-cysteine
-
Triticum aestivum
4.3.3.7
threo-4-hydroxy-L-lysine
-
Triticum aestivum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.3.7
0.8
-
L-aspartate-4-semialdehyde
-
Triticum aestivum
4.3.3.7
11.7
-
pyruvate
-
Triticum aestivum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.3.7
123000
-
gel filtration
Triticum aestivum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
Triticum aestivum
biosynthesis of lysine via the diaminopimelate pathway
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.3.7
-
Triticum aestivum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.3.3.7
cell suspension culture
-
Triticum aestivum
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.3.3.7
additional information
-
-
Triticum aestivum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
D-isomer inactive
33902
Triticum aestivum
dihydrodipicolinate + H2O
-
33902
Triticum aestivum
?
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
biosynthesis of lysine via the diaminopimelate pathway
33902
Triticum aestivum
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.3.3.7
tetramer
4 * 32000-34000, SDS-PAGE
Triticum aestivum
Temperature Stability [░C] (protein specific)
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
4.3.3.7
60
-
1 min, 50% loss of activity, 5 min, complete inactivation
Triticum aestivum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3.3.7
8
-
-
Triticum aestivum